Sequences from confirmation amplicon


Confirmation sequences for line 228D02 and hit At3g16650 are shown.
Line ID 228D02
Locus At3g16650 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:5671563
Primers used for PCR CK06 - 8474, show primer details
   
Sequence Name 11-K017714-022-228-D02C-CK06
GenBank Accession LN490901 [GenBank]
Sequencing primer gene specific primer, CK06: 5'-TTTGTCTCACAACAGGAAAAACAG-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K017714-022-228-D02C-CK06
TACCTGGAAGCGCAAGGGCTTTCAATGGAGCCGTCTTTTCACTGTTATGGTCAGCAATAC
GTGTAGGCTTACTTGCAGGTTCTACGCCTCCAAATGCAACTTGAATCTATACAAGTAACG
TCCAAGTCATTAACTTTTATATTCTACTTGATGTTCACTAAAACGCTTGATGCAAACTAA
ACATCTTACTATAAAGATGAGGGTAATATGCTAGAAAGCAAACTCAAACAATAGTAGCTG
ATGAGAAGAATCTAACTCCAGATAAAAGGTAAACTATAAAAGTGATAAGCATGGATCAGC
AATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATT
CAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACA
AATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAAT
CTTTATTTCGACGTGTCTACATT
   
Sequence Name 12-K017714-022-228-D02C-8409
GenBank Accession LN491359 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K017714-022-228-D02C-8409
CATGCTTATCACTTTTATAGTTTACCTTTTATCTGGAGTTAGATTCTTCTCATCAGCTAC
TATTGTTTGAGTTTGCTTTCTAGCATATTACCCTCATCTTTATAGTAAGATGTTTAGTTT
GCATCAAGCGTTTTAGTGAACATCAAGTAGAATATAAAAGTTAATGACTTGGACGTTACT
TGTATAGAATTCAAGTTGCATTTGGAGGCGTAGAACCTGCAAGTAAGCCTACACGTATTG
CTGACCATAACAGTGAAAAGACGGCTCCATTGAAAGCCCTGGGCTTTCCAGGAAGTCCTT
TATCCGGTTTTTCCGGTGGGAAAACAA

Last Updated on 06.12.2018 16:11