Sequences from confirmation amplicon


Confirmation sequences for line 229D04 and hit At4g16760 are shown.
Line ID 229D04
Locus At4g16760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:9425159
Primers used for PCR 14hw - 8474, show primer details
   
Sequence Name 40-K112831-0022-229-D04C-14hw
GenBank Accession LN503762 [GenBank]
Sequencing primer gene specific primer, 14hw: 5'-GATTCTCCCTCTCAATGTCAGTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K112831-0022-229-D04C-14hw
CGAACTGGAGCAGACATAGGTGGCAAAGGAGTGAAGAAACAGCTGAATAATCTGTGTTAC
ATTTATGCTCTTTATCTCCTCCACAAACATCTCGGCGATTTCCTCTCCACTAACTGCATC
ACTCCCAAACAAGCCTCTCTTGCTAACGACCAGCTCCGTTCCTTATACACTCAGGTTCTT
TCTTCCTCTCTCTCTCCATCTCTGCATTTGATTCCTTTTTAAGCTAAGTTTTAACACGTT
TTTGTGGCATTAGGACACGTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGA
TGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAG
ATGTCCGCAGCGTTATA
   
Sequence Name 11-K112829-0022-229-D04C-8409
GenBank Accession LN491191 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K112829-0022-229-D04C-8409
CGTGTTAAACTTAGCTTAAAAAGGAATCAAATGCAGAGATGGAGAGAGAGAGGAAGAAAG
AACCTGAGTGTATAAGGAACGGAGCTGGTCGTTAGCAAGAGAGGCTTGTTTGGGAGTGAT
GCAGTTAGTGGAGAGGAAATCGCCGAGATGTTTGTGGAGGAGATAAAGAGCATAAATGTA
ACACAGATTATTCAGCTGTTTCTTCACTCCTTTGCCACCTATGTCTTGCTCCAGTTTCGC
TATGAACCTAACCAAGACAAAGATCACAAAATTCAGGAACTGACATTGAGAGGGAGAAT

Last Updated on 06.12.2018 16:11