Sequences from confirmation amplicon


Confirmation sequences for line 229E02 and hit At5g12870 are shown.
Line ID 229E02
Locus At5g12870 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:4063238
Primers used for PCR DR08 - 8474, show primer details
   
Sequence Name 02-K018975-022-229-E02C-DR08
GenBank Accession LN486838 [GenBank]
Sequencing primer gene specific primer, DR08: 5'-GACTAATCATTACATGGCACCTCA-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K018975-022-229-E02C-DR08
ATTCTACGATTACTCTTGAAAACATCAAACCAGAACTTTCTGAATACAATATACTCTCTA
ACAATGTATATATATTTGCATTTATGTGTGTGTGGCTAAGAATCTTGAGTATAAACCGCA
ATAGAAAAAAGAATAAGATGACGTAAAAATATTCATATGCTTTGTTTGAAGTTGAAGTAA
AACGAAGGAACCTCAGTGTTCATCAACTGGTCAAGGTCCCAAAATTCTTCCACTGGTCTC
ATACCCTCAGGTGCGTTTCCGGATCCATTAGTGTTCAACTCGTCTAAGTGGTTGTTTATA
TTTGCATTGTACCAATCACCTTCTTCACATTCCAAAGGTGGGAAGTAGAGTTCCCCTTGT
ACCCCTGCGTTTACTCCATAGATACCCATCTAC
   
Sequence Name 06-K018975-022-229-E02C-8409
GenBank Accession LN488606 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K018975-022-229-E02C-8409
TAGATTTCCCGGACATGAAGCCAACGGGAAACATGATCAGCCACCCGTGCAATGACGATT
TTACCCCTTATGTAGATGGTATCTATGGAGTAAACGCAGGGGTACAAGGGGAACTCTACT
TCCCACCTTTGGAATGTGAAGAAGGTGATTGGTACAATGCAAATATAAACAACCACTTAG
ACGAGTTGAACACTAATGGATCCGGAAACGCACCTGAGGGTATGAGACCAGTGGAAGAAT
TTTGGGACCTTGACCAGTTGATGAACACTGAGGTTCCTTCGTTTTACTTCAACTTCAAAC
AAAGCATATGAATATTTTTACGTCATCTTATTCTTTTTTCTATTGCGGTTTATACTCAAG
ATTCTTAGCCACACACACATAAATGCAAATATATATACATTGTTAGAGAGTATTTTGTAT
TTCGAATAATCTTTTCGTACTAGGGCTTGAGCCTTGAGGTGCCATG
Primers used for PCR FN68 - 3144, show primer details
   
Sequence Name 18-K032733-022-229-E02C-3144
GenBank Accession LN494198 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K032733-022-229-E02C-3144
TCCACTATCCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGAC
AGGTTACCCGGGGATCAGATTGTCATTTCATTTATCTAAATTATCATTGGTAATTACTCT
TTCTTTTTCTCCATGTTGGTTTGTATGTGGGTCAAGGAAGGGTTGACGAAGCCTTGTTCT
TGTAAGGACATGAAGCTTCCTATAATGTCTTTAATATCCAAAGAAGATGCGGAATTAGAA
GAGGAATCGCTTGCTGTGTTGGGTGATGAGGATGAGTTGTTGATTAAGTTGGAGGTATCG
GACATCTTCTTTAGCCTTTTCTTTATTGTTGAGTTCCAGAAATTCTTGATCTCGTTATCG
GTCCGACCAGGCAATCGTGCTGCAATCTGAGACCACCTATGAAATCGAAGAATAAGAGAG
TAGAATAGTTTAATGAGATGATGTTATGTAAAATGTATACCAGTGCATGGTTTGCATGGG
AATTAGAGAGTGATCCGTTCTTCAATGTT
   
Sequence Name 50-K032733-022-229-E02C-FN68
GenBank Accession LN507073 [GenBank]
Sequencing primer gene specific primer, FN68: 5'-AAAACATGAAGAACGGATCACTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K032733-022-229-E02C-FN68
GTAACATTTTACATAACATCATCTCATTAAACTATTCTACTCTCTTATTCTTCGATTTCA
TAGGTGGTCTCAGATTGCAGCACGATTGCCTGGTCGGACCGATAACGAGATCAAGAATTT
CTGGAACTCAACAATAAAGAAAAGGCTAAAGAAGATGTCCGATACCTCCAACTTAATCAA
CAACTCATCCTCATCACCCAACACAGCAAGCGATTCCTCTTCTAATTCCGCATCTTCTTT
GGATATTAAAGACATTATAGGAAGCTTCATGTCCTTACAAGAACAAGGCTTCGTCAACCC
TTCCTTGACCCACATACAAACCAACATGGAGAAAAAGAAAGAGTAATTACCAATGATAAT
TTAGATAAATGAAATGACAATCTGATCCCCGGGTAACCTGTCCTCTCCAAATGAAATGAA
CTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACG
TCAGTGGAGATATCACATCAATCCACTTGCTTTGAAGACGTGGTTGGAACGTCTTCTTTT
TCCACGATGCTCCTCGTGGTG

Last Updated on 06.12.2018 16:11