Sequences from confirmation amplicon


Confirmation sequences for line 229E08 and hit At1g77610 are shown.
Line ID 229E08
Locus At1g77610 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:29166821
Primers used for PCR RK57 - 8474, show primer details
   
Sequence Name 23-K018967-022-229-E08C-RK57
GenBank Accession LN496364 [GenBank]
Sequencing primer gene specific primer, RK57: 5'-TATCAACCATGAGACCATTACAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K018967-022-229-E08C-RK57
CCTGCCAATCATATTACAAACATGAGAGTGAGTGTCCAAGGCAACAAACAAATCACAAGT
TGGTGATGACAAAAACCATAGCAATGTGCCTCGAAAATTTGATATTCTAGTGGTGATTAA
CTTGTTTAAAACAAACTTATATGGATACAGTCATGCCCTGACCACTACGAATAAGACGTA
TAATGGAATATGAAGTACGCTTAAGACGTAGATGCGAAGAAACTCTAGCTGACAGGCTTT
CTATTTCTTTTACCATGGTAAGATAGGTAAATTGATAAGAACTTAGTAATGCCAGTATAA
TGAATTAATAGTGCTTATCCTTTTGTTGGTTTGGACCATCCAGAGCAATCCGAAAGAAAA
ATTTAGTAACGGGTTTAGGGTTGTCTTAGTTACCTTAAGGTTTCCAGCCACGTTGAATGT
GACTGCAGTTGTGGAGTGAATGTGACTGCAGGATATATTCAATTGTAAATGGCTTCATGT
CCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAG
TAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGA
AAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAA
ACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACA
   
Sequence Name 24-K018967-022-229-E08C-8409
GenBank Accession LN496805 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K018967-022-229-E08C-8409
CCATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGCAGTCACATTC
ACTCCACAACTGCAGTCACATTCAACGTGGCTGGAAACCTTAAGGTAACTAAGACAACCC
TAAACCCGTTACTAAATTTTTCTTTCGGATTGCTCTGGATGGTCCAAACCAACAAAAGGA
TAAGCACTATTAATTCATTATACTGGCATTACTAAGTTCTTATCAATTTACCTATCTTAC
CATGGTAAAAGAAATAGAAAGCCTGTCAGCTAGAGTTTCTTCGCATCTACGTCTTAAGCG
TACTTCATATTCCATTATACGTCTTATTCGTAGTGGTCAGGGCATGACTGTATCCATATA
AGTTTGTTTTAAACAAGTTAATCACCACTAGAATATCAAATTTTCGAGGCACATTGCTAT
GGTTTTTGTCATCACCAACTTGTGATTTGTTTGTTGCCTTGGACACTCACTCTCATGTTT
GTAATATGATTTGCAGGTTGCAGTGGCTGTAATGGTCT

Last Updated on 06.12.2018 16:11