Sequences from confirmation amplicon


Confirmation sequences for line 229F03 and hit At2g34880 are shown.
Line ID 229F03
Locus At2g34880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:14714415
Primers used for PCR PC60 - 8474, show primer details
   
Sequence Name 81-K103792-0022-229-F03C-8409
GenBank Accession LN514060 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>81-K103792-0022-229-F03C-8409
GTCTTTCAAGAAGTATGAAAGGGCANCATATGGGTCTTCAGGATGAAGATGATAATTCGT
ATATTGTTTTGTGCATTTGAAGAATCAGCAAAAATCAAGGAAGAAAATGGCTGGTTGGTG
GATAGTGGTTTCATAAACCACATGACCAAGGAAGTGAAAAAAAGTTCACAAGGCTAGAAA
GAAGTATTAACGTTCCAATTCAAAATTGGAAACGGGGGAAAAATGAAATTCAACCACAAG
AGTTGAAGAAATGAGTAAAAAGTTTGGTGTGAGATCTAAAACTCAATTAAGAGGGAGGGT
TAGTTTATAATTGAGTTTGGTCTAAATGTGATTTAATTATGTAATTAAGTATGTTTGTTA
AGTTTATACAATCAAGAGTCTTGTCTTAAGGGTAGTTATGTTAAGAGTCATAAGTGAAGA
GATTTTCAATTAAAGAGGTTTCAGTTTTCCAGATTTAAAAAAAAAAAAAAAAAAGAAAAA
AAAAAAAAAAAAA
Primers used for PCR 29dy - 3144, show primer details
   
Sequence Name 21-K118238-0022-229-F03C-29dy
GenBank Accession LN495854 [GenBank]
Sequencing primer gene specific primer, 29dy: 5'-CAAATCGTCCAAAACATTCTCATA-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K118238-0022-229-F03C-29dy
ATGGAACAACGAAGGATTTTACCATACTGCCTGTGACAGAGTTGATAGGGACTCTTCAAG
CGGATCAGAATCGTGTATCTTCAAGGAATGAGAACACGTCCGAAGGTGTGTTTCATGCTA
GAAAAAGTGGAGGGAATCCTGGTTTTAAAGCAGAAGATGAACAAAAGTGGTGTGGGTTTT
GTAAGAAACAGGGGATGGCTTATTGGCAAAAAGGTTCAAGGAATCAAAAAGAATGTTACA
ACTGTGGGAACACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTA
CCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATA
GTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCACATCAATCCA
   
Sequence Name 07-K118237-0022-229-F03C-35St
GenBank Accession LN489469 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K118237-0022-229-F03C-35St
AGACCCTTCCTCTATATAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCCGGGGATC
AGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTCCCACAGTTGTAACATTCTT
TTTGATTCCTTGAACCTTTTTGCCAATAAGCCATCCCCTGTTTCTTACAAAACCCACACC
ACTTTTGTTCATCTTCTGCTTTAAAACCAGGATTCCCTCCACTTTTTCTAGCATGAAACA
CACCTTCGGACGTGTTCTCATTCCTTGAAGATACACGATTCTGATCCGCTTGAAGAGTCC
CTATCAACTCTGTCACAGGCAGTATGGTAAAATCCTTCGTTTGTTCCATCACTGCCACTA
TGCTATCGAACCTCGCCGGGAGGGATATGAGAATGTTTGGNACGATTTG

Last Updated on 06.12.2018 16:11