Sequences from confirmation amplicon


Confirmation sequences for line 230A02 and hit At5g59120 are shown.
Line ID 230A02
Locus At5g59120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:23864911
Primers used for PCR CR15 - 8474, show primer details
   
Sequence Name 47-K017402-022-230-A02C-CR15
GenBank Accession LN506035 [GenBank]
Sequencing primer gene specific primer, CR15: 5'-GTCGCTCTGTTCTTATTTGGTTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K017402-022-230-A02C-cr15
AATGCTACTGGAACTGGCATCGCATCGACAGAGTTTGCTTATGGATCTGGACATGTCGAC
CCGATAGCCGCTTCAAATCCTGGACTTGTCTATGAATTAGACAAATCAGACCACATCGCC
TTTCTCTGCGGCATGAACTACACTTCGCAAGTCCTGAAAGTCATCTCTGGTGAAACCGTC
ACTTGCTCCGAAGCAAAAAAAATCCTACCAAGAAACCTCAACTATCCTTCAATGTCTGCC
AAATTGTCAGGATCTGGTACCACCTTCACTGTAACGTTCAACAGAACCTTGACTAATGTT
GGCACGCCAAACTCTACATACACATCAAAGGTAGTGGCGGGTCACGGATCTAAGCTCGAC
GTTAAGATCACACCTAGTGTTCTGTCTTTTAAGACTGTGAACGAGAAGCAATCTTTTACG
GTTACTGTTACCGGCAGCAATCTCGACTCTGAAGTGCCTTCGTCTGCAAATCTGATCTGG
TCTGACGGTACCCATAACGTGAGAAGTCCCATTGTTGTTTATATTCAATTGTAAATGGCT
TCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATA
AAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAG
CAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACA
   
Sequence Name 48-K017402-022-230-A02C-8409
GenBank Accession LN506364 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K017402-022-230-A02C-8409
ACATGAAGCCATTTACAATTGAATATAAACAACAATGGGACTTCTCACGTTATGGGTACC
GTCAGACCAGATCAGATTTGCAGACGAAGGCACTTCAGAGTCGAGATTGCTGCCGGTAAC
AGTAACCGTAAAAGATTGCTTCTCGTTCACAGTCTTAAAAGACAGAACACTAGGTGTGAT
CTTAACGTCGAGCTTAGATCCGTGACCCGCCACTACCTTTGATGTGTATGTAGAGTTTGG
CGTGCCAACATTAGTCAAGGTTCTGTTGAACGTTACAGTGAAGGTGGTACCAGATCCTGA
CAATTTGGCAGACATTGAAGGATAGTTGAGGTTTCTTGGTAGGATTTTTTTTGCTTCGGA
GCAAGTGACGGTTTCACCAGAGATGACTTTCAGGACTTGCGAAGTGTAGTTCATGCCGCA
GAGAAAGGCGATGTGGTCTGATTTGTCTAATTCATAGACAAGTCCAGGATTTGAAGCGGC
TATCGGGTCGACATGTCCAGATCCATAAGCAAACTCTGTCGATGCGATGCCAGTTCCAGT
AGCATTAACAGGCCAAGCTGCAAATATTGGTTAAGAAACCAAATAA

Last Updated on 06.12.2018 16:11