Sequences from confirmation amplicon


Confirmation sequences for line 230F08 and hit At5g13490 are shown.
Line ID 230F08
Locus At5g13490 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:4336809
Primers used for PCR MN44 - 8474, show primer details
   
Sequence Name 21-K031754-022-230-F08C-8409
GenBank Accession LN495552 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K031754-022-230-F08C-8409
TGAAGGCAGTTCAATGGTCTTGTTGATGTCTACAAGAAGACCCTCAAGTCAGATGGTATT
GCTGGACTTTACCGTGGTTTCAACATCTCGTGTGCTGGTATCATTGTCTACCGTGGTCTC
TACTTTGGACTGTACGACTCTGTGAAGCCTGTCCTCCTCACTGGAGACCTGCAGGTTTGT
TTGAGCTCTATTCTTATATATCTGTAAGGTGACAGCTTAAGAAATCAGGGATCCTGATTC
TAATGTTGTTATTTCTTTCCTTGCTGGATATTTCAGGACAGTTTCTTCGCTAGTTTTGCT
   
Sequence Name 22-K031754-022-230-F08C-MN44
GenBank Accession LN495998 [GenBank]
Sequencing primer gene specific primer, MN44: 5'-GAGCAAAACTAGCGAAGAAACTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K031754-022-230-F08C-MN44
CATTAGAATCAGGATCCCTGATTTCTTAAGCTGTCACCTTACAGATATATAAGAATAGAG
CTCAAACAAACCTGCAGGTCTCCAGTGAGGAGGACAGGCTTCACAGAGTCGTACAGTCCA
AAGTAGAGACCACGGTAGACAATGATACCAGCACACGAGATGTTGAAACCACGGTAAAGT
CCAGCAATACCATCTGACTTGAGGGTCTTCTTGTAGACATCAACAAGACCATTGAACTGC
CTTTCACCTCCTCCCTTCTTGGCAGACTTGGAGTCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
TGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCG
TCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGAC
GTGTCTACATTCACGTCCAAATGG

Last Updated on 06.12.2018 16:11