Sequences from confirmation amplicon


Confirmation sequences for line 230H01 and hit At5g06530 are shown.
Line ID 230H01
Locus At5g06530 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:1992917
Primers used for PCR 20sm - 8474, show primer details
   
Sequence Name 13-K116987-0022-230-H01C-20sm
GenBank Accession LN492219 [GenBank]
Sequencing primer gene specific primer, 20sm: 5'-TCCACAGATGAAGTCAATTTCTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K116987-0022-230-H01C-20sm
ATCATTTCCAAAAACCACTACTAAGTCTTGAGATTATATATACATAACTCATGAAAATGA
AACCTATTTAAAAAAATAAAAAGATCGTATTACACATTTTCGCTTCTTTTCCCCCCTTAA
TTAGTCTAATCTTAACTATGGATATCTTAAGTCTTGTACATAAATTTCATGTAAGATGAG
ATTATGTACAAGACCTGAAGCTGATGAAAAGTAGATACACAGAGATAGAGACAAATGTGT
GATATTCTTTTGATCTCCTCTCAGTGCCTGTGCAAACAGAGAAGATCAGTACCAAAGAGA
TCATTCCCATACACGAAAACCACTAGCTACTTACTATACTTCAGTCCAATTTCTAACATT
TCTTTTGCTCTTTTATTGTTTTGGTTTGATATAATTATTTACCTTGAGAAAGATGGGCAA
TGTTGGTTCTGCTTGAAACTTAGGCTTCTTCTTTCCCGCTTCAATATCTACATGTTACGT
TACGTTGGTTCTGTATATATTCAGTTTAGGATATATTTAGGATATATTCAGTTTTGGTAA
AAGATGGGCAATGTTGGTTCTGTTTCAGGATATATTCAATTGTAAATGGCTTCATGTCCG
GGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAA
TTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 01-K116986-0022-230-H01C-8409
GenBank Accession LN486745 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K116986-0022-230-H01C-8409
ATCCTGAAACAGAACCAACATTGCCCATCTTTTACAAAACTGAATATATCCTAAATATAT
CCTAAACTGAATATATACAGAACCAACGTAACGTAACATGTAGATATTGAAGCGGGAAAG
AAGAAGCCTAAGTTTCAAGCAGAACCAACATTGCCCATCTTTCTCAAGGTAAATAATTAT
ATCAAACCAAAACAATAAAAGAGCAAAAGAAATGTTAGAAATTGGACTGAAGTATAGTAA
GTAGCTAGTGGTTTTCGTGTATGGGAATGATCTCTTTGGTACTGATCTTCTCTGTTTGCA
CAGGCACTGAGAGGAGATCAAAAGAATATCACACATTTGTCTCTATCTCTGTGTATCTAC
TTTTCATCAGCTTCAGGTCTTGTACATAATCTCATCTTACATGAAATTTATGTACAAGAC
TTAAGATATCCATAGTTAAGATTAGACTAATTAAGGGGGGAAAAGAAGCGAAAATGTGTA
ATACGATCTTTTTATTTTTTTAAATAGGTTTCATTTTCATGAGTTATGTATATATAATCT
CAAGACTTAGTAGTGGTTTTTGTGAAATTGATGCAGTTCAGGGATGTTACATACAAAGTG
GTGATCAAGAAATTGACTTCATCCTGTGGA

Last Updated on 06.12.2018 16:11