Sequences from confirmation amplicon


Confirmation sequences for line 230H02 and hit At2g32340 are shown.
Line ID 230H02
Locus At2g32340 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:13733369
Primers used for PCR 04rl - 8474, show primer details
   
Sequence Name 68-K115895-0022-230-H02C-04rl
GenBank Accession LN512077 [GenBank]
Sequencing primer gene specific primer, 04rl: 5'-AGAGGAAGTGGTCGATTAATCTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K115895-0022-230-H02C-04rl
ACTTATCTCGCTCATGCACAGTGTCTCCATGAGAGTCGGAAATTCCGTGCTCATTTCTTG
AATCGCCAATGTCAGCATATCCCCATCGCCCAGGTCTTTCAGCTAAAGCCATCCAAACTT
ATCATCACTCAATATTTCCTCTGACTGAATCACAAAAATGGAAAAAAAGAAGGAAGAAGA
AAAGACTTTACCATCTTCTCAAGTTCCTTAGGGTTCGGCAGAAAGACAGCTTGACTTACT
ATGCTATACACTAGTTTTATTTTATGCCATAGAGGCATCTTACCCCATGTTCTCTGTAAC
GTGATCTGCTTCGACAAATATGAAAGAAACTGAATACTCACTAGAACACAGTATAAAAAA
CAGAGATGCACTTCATAACATTCCTATAAGAAAGGTTTGAATTATGAACACCAAAGTCAC
CTGTACTGAGCGATCGCCAAGAAACACTCTGCCACCATATTTGTTTGCCTCTTCAATTGT
AAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGG
AGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCG
TTATT
   
Sequence Name 38-K115894-0022-230-H02C-8409
GenBank Accession LN503106 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K115894-0022-230-H02C-8409
TTTCATTGAGAGGCAACAATATGGTGGCAGAGTGTTTCTTGGCGATCGCTCAGTACAGGT
GACTTTGGTGTTCATAATTCAAACCTTTCTTATAGGAATGTTATGAAGTGCATCTCTGTT
TTTTATACTGTGTTCTAGTGAGTATTCAGTTTCTTTCATATTTGTCGAAGCAGATCACGT
TACAGAGAACATGGGGTAAGATGCCTCTATGGCATAAAATAAAACTAGTGTATAGCATAG
TAAGTCAAGCTGTCTTTCTGCCGAACCCTAAGGAACTTGAGAAGATGGTAAAGTCTTTTC
TTCTTCCTTCTTTTTTTCCATTTTTGTGATTCAGTCAGAGGAAATATTGAGTGATGATAA
GTTTGGATGGCTTTAGCTGAAAGACCTGGGCGATGGGGATATGCTGACATTGGCGATTCA
AGAAATGAGCACGGAATTTCCGACTCTCATGGAGACACTTGTGCATGAGCGAGATAAGTT
AGTATACTATCCTCTCTAATATAGAGTTTACAAGATTAATCGACCACTTCCTCTA

Last Updated on 06.12.2018 16:11