Sequences from confirmation amplicon


Confirmation sequences for line 231B05 and hit At1g63400 are shown.
Line ID 231B05
Locus At1g63400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:23508497
Primers used for PCR ZZ30 - 8474, show primer details
   
Sequence Name 10-K020358-022-231-B05C-8409
GenBank Accession LN490451 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K020358-022-231-B05C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTTACATGTTTGCAAGGAC
TGTTTCCCAAACGTAGTGACTTACAATACTCTTATTAACGGATTTTGTAAGGCTAAGAGA
ATAGACGAAGGTGTGGAACTCTTCCGCGAGATGTCTCAAAGAGGATTGGTTGGCAACACA
GTCACTTACACCACTCTTATCCATGGATTTTTTCAAGCTAGAGATTGTGATAATGCCCAA
ATGGTGTTCAAACAGATGGTTTCTGATGGTGTGCATCCCAATATTATGACATACAACACT
CTGTTAGATGGACTTTGTAAAAATGGGAAGCTAGAGAAAGCAATGGTTGTATTCGAGTAT
CTGCAAAGGAGTAAAATGGAACCTACTATTTACACATATAATATTATGATTGAAGGGATG
TGCA
   
Sequence Name 14-K020358-022-231-B05C-ZZ30
GenBank Accession LN492306 [GenBank]
Sequencing primer gene specific primer, ZZ30: 5'-TTGCACATCCCTTCAATCATAATA-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K020358-022-231-B05C-ZZ30
TATGTGTAAATAGTAGGTTCCATTTTACTCCTTTGCAGATACTCGAATACAACCATTGCT
TTCTCTAGCTTCCCATTTTTACAAAGTCCATCTAACAGAGTGTTGTATGTCATAATATTG
GGATGCACACCATCAGAAACCATCTGTTTGAACACCATTTGGGCATTATCACAATCTCTA
GCTTGAAAAAATCCATGGATAAGAGTGGTGTAAGTGACTGTGTTGCCAACCAATCCTCTT
TGAGACATCTCGCGGAAGAGTTCCACACCTTCGTCTATTCTCTTAGCCTTACAAAATCCG
TTAATAAGAGTATTGTAAGTCACTACGTTTGGGAAACAGTCCTTGCAAACATGTAAGGAT
ATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGA
TGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAG
ATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCC
GTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGA
CGTGTCTACATTCA

Last Updated on 06.12.2018 16:11