Sequences from confirmation amplicon


Confirmation sequences for line 231F05 and hit At3g61790 are shown.
Line ID 231F05
Locus At3g61790 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:22873358
Primers used for PCR IF59 - 8474, show primer details
   
Sequence Name 34-K101501-0022-231-F05C-8409
GenBank Accession LN501354 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K101501-0022-231-F05C-8409
TTCGAGAGCTCATGAACACTGGTGGTGGTAGGGAGAAGCCCAGAAGCAGCAGCATTAACG
TTGGTGGTGTTGTTATTAGTATGATGATGCTTGGAAACAGAAGCGTAGGAATGGCGATCT
TGGTGGATCTCTTCATCATCTGTCACATCCATGGTTGATGTACAGTCCATGCTATCCAAA
TCCATTGAAGAAAGGTGGAGACTTTGATTGATCTTCGATCGAAACGAAAGATCAAAATCC
GGAATTGAAGAAACACCACCGAAACAGGTCAACCCTCGTGAAGTGTTGTTAATTT
   
Sequence Name 34-K101505-0022-231-F05C-IF59
GenBank Accession LN501355 [GenBank]
Sequencing primer gene specific primer, IF59: 5'-AAAATTAACAACACTTCACGAGGG-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K101505-0022-231-F05C-IF59
TCATTCCGGATTTTGATCTTTCGTTTCGATCGAAGATCAATCAAAGTCTCCACCTTTCTT
CAATGGATTTGGATAGCATGGACTGTACATCAACCATGGATGTGACAGATGATGAAGAGA
TCCACCAAGATCGCCATTCCTACGCTTCTGTTTCCAAGCATCATCATACTAATAACAACA
CCACCAACGTTAATGCTGCTGCTTCTGGGCTTCTCCCTACCACCACCAGTGTTCATGAGC
TTCTCGAATGTCCTGTCTGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATAT
GGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC

Last Updated on 06.12.2018 16:11