Sequences from confirmation amplicon


Confirmation sequences for line 232A01 and hit At3g26780 are shown.
Line ID 232A01
Locus At3g26780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:9849002
Primers used for PCR MRA1 - 8474, show primer details
   
Sequence Name 43-K019550-022-232-A01C-MRA1
GenBank Accession LN504684 [GenBank]
Sequencing primer gene specific primer, MRA1: 5'-CAACCATGAACAAGATAAGCTCAC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K019550-022-232-A01C-MRA1
CAGTAACAACAGTACTTAGTGGTTTCAATGGATTCATTAGATCAATCAACTCTTACTTAA
TTCATTCCCAAGATATTACCACAGATCAATCTCACACAAATTAGGCAACATTGTACACTT
CGTTAAACCTTAGATCTAAGAGAAGAAACTTTACCTGACAAACAGAAACAGCCATGGATC
TAGCTCGATCTAGAGGTGACGAATAAACCGAATTAAACCGAACACCTTGAGATTTGAAGA
AAACAGCAAGAGCCCTAGCTTGACGTTTACCATTAGCAGTAAGAGCAGCTACATGACATC
TCCCACCAATCAAATCAGGTTTAAGATTAAGATCAGACTCACCATGGCTAATCAGATAAA
CCTCTGTCACAGCTCGATCTTCATCACCAGACACGATTCGAGAGAAGATAGACGGCGGTT
GCGGCGGCGGAGAGAGGACATGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGC
AATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATT
CAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACA
AATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAAT
CTTTATTTCGACGTGTCTAC
   
Sequence Name 44-K019550-022-232-A01C-8409
GenBank Accession LN505036 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K019550-022-232-A01C-8409
CATGTAGATTTCCCGGACATGAAGCCATTTACATGTCCTCTCTCCGCCGCCGCAACCGCC
GTCTATCTTCTCTCGAATCGTGTCTGGTGATGAAGATCGAGCTGTGACAGAGGTTTATCT
GATTAGCCATGGTGAGTCTGATCTTAATCTTAAACCTGATTTGATTGGTGGGAGATGTCA
TGTAGCTGCTCTTACTGCTAATGGTAAACGTCAAGCTAGGGCTCTTGCTGTTTTCTTCAA
ATCTCAAGGTGTTCGGTTTAATTCGGTTTATTCGTCACCTCTAGATCGAGCTAGATCCAT
GGCTGTTTCTGTTTGTCAGGTAAAGTTTCTTCTCTTAGATCTAAGGTTTAACGAAGTGTA
CAATGTTGCCTAATTTGTGTGAGATTGATCTGTGGTAATATCTTGGGAATGAATTAAGTA
AGAGTTGATTGATCTAATGAATCCATTGAAACCACTAAGTACTGTTGTTACTGCTCTTTG
TGTGAGCTTATCT

Last Updated on 06.12.2018 16:11