Sequences from confirmation amplicon


Confirmation sequences for line 232B02 and hit At4g23150 are shown.
Line ID 232B02
Locus At4g23150 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:12126824
Primers used for PCR MRB2 - 8474, show primer details
   
Sequence Name 79-K021740-022-232-B02C-8409
GenBank Accession LN513757 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>79-K021740-022-232-B02C-8409
CATTTACCATCTAATGCAGGTGTTTCATTTAAGGACTTGAATCTTTACCATCTAAAGCAG
GTGTTGTGCCATAAGTCTTCTTTGCCCTCTTTGCAAAGAAACAATAACCAGCTATGAAAA
TCAGAACAGCGACTATGATAGGCACAACAACGGCTACCACGACCACATTTGAATTCCCAC
TTTTATCTGCGTTTAAACAGATCATCTGCTTTAGTAAGAAAATAGTAGACTTATAACAAA
ACAAAAATTGAAAACTCTGCGTTCCATATTGGTAATACTGCAAAGATCAAGTAAACAGGG
CAAAGGTAGAAGAAACTGTTCACCAGATAGTGAAGGAGATGCCAACGGAGGCAGCGGCGG
TGGTGGTGTACCAATGGCGGTTTCGTTGTAGAAATCGTAAAGCTCGTACCTTGAATTACA
ACTTGGCCAAAACTGTCTTGCTCCAATTCTAGAAAATGGCATTCTATTGATGGAACTTGT
CAGACAGTTCATGCAGTTTTGTCTTGAAAGATCAGGAGTGCATTGAACAAGCCCGTAGAA
AGTCTGGAGTGCGGTCAATTCGGTCTTTATCGTAGAGAATTTTCTAGGATTGTCGGCTGC
TTCGATGGCAATTTGGTTCATATTAGATAACACCAAATTAGTAAACTGGTTTATTTGATT
TTGAATAGGAGAAATATGATTGGGGTTCCTCAAGATAAATTCTCCTTCGTTTGTAATGGC
CGTCGATAGAATATTCTTGTGAGAGTATCTGAGTATGCACTCCTCGTAATAGAACACAGC
TTCTCTCTGATTC
   
Sequence Name 80-K021740-022-232-B02C-MRB2
GenBank Accession LN513887 [GenBank]
Sequencing primer gene specific primer, MRB2: 5'-ACCTTTAGTAGGTGTCCGAATCAG-3'
Sequence (A. th genome BLAST matches underlined) 
>80-K021740-022-232-B02C-mrb2
CATACTCAGATACTCTCACAAGAATATTCTATCGACGGCCATTACAAACGAAGGAGAATT
TATCTTGAGGAACCCCAATCATATTTCTCCTATTCAAAATCAAATAAACCAGTTTACTAA
TTTGGTGTTATCTAATATGAACCAAATTGCCATCGAAGCAGCCGACAATCCTAGAAAATT
CTCTACGATAAAGACCGAATTGACCGCACTCCAGACTTTCTACGGGCTTGTTCAATGCAC
TCCTGATCTTTCAAGACAAAACTGCATGAACTGTCTGACAAGTTCCATCAATAGAATGCC
ATTTTCTAGAATTGGAGCAAGACAGTTTTGGCCAAGTTGTAATTCAAGGTACGAGCTTTA
CGATTTCTACAACGAAACCGCCATTGGTACACCACCACCGCCGCTGCCTCCGTTGGCATC
TCCTTCACTATCTGGTGAACAGTTTCTTCTACCTTTGCCCTGTTTACTTGATCTTTGCAG
TATTACCAATATGGAACGCAGAGTTTTCAATTTTTGTTTTGTTATAAGTCTACTATTTTC
TTACTAAAGCAGATGATCTGTTTAAACGCAGATAAAAGTGGGAATTCAAATGTGGTCGTG
GTAGCCGTTGTTGTGCCTATCATAGTCGCTGTTCTGATTTTCATAGCTGGTTATTGTTTC
TTTGCAAAGAGGGCAAAGAAGACTTATGGCACAACACCTGCTTTAGATGGTAAAGATTCA
AGTCCTTAAATGAAACACCTGCATTAGATGGTAAATGGCTTCATGTCCGGGAAATCTACA
TGGATCAGCAATGAGTATGATGGTCAATATGGAGAANAAGAAAGAGTAATTACCAATTTT
TTTTCAATTCANAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGAN
TAAACGACAAATTACGATCCGTCG

Last Updated on 06.12.2018 16:11