Sequences from confirmation amplicon


Confirmation sequences for line 232C03 and hit At3g24225 are shown.
Line ID 232C03
Locus At3g24225 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:8768899
Primers used for PCR CL02 - 8474, show primer details
   
Sequence Name 64-K021739-022-232-C03C-CL02
GenBank Accession LN511103 [GenBank]
Sequencing primer gene specific primer, CL02: 5'-GGAAAGGCAAAGAATATCGAGTAA-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K021739-022-232-C03C-CL02
AGAACATAGATACACATATAATTGTTCTTCAATTACCACAGTTACCTGTTGTGGAGTGGA
TTTGGACCAGTGGGAATTACCCGTTTTGAGTCTAAAGCAGAAGAGTTAGCGATCGTTCCC
ATGGAGTCATACTTTAGAACTTGTTCTTCTTCTTCATATGATCCATTATTCATCAGCAAA
CTCCGCATTGAAGCTGATTCCGACTGATGAATGAAGGCAAGAATCAGGAGAGAAGAAGCC
AATATCATCAAACCCTTTATCTTCATTTGTCTATTTTTGGTCAAATATTGTTTTTGTTTG
TTTGATGGGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGAC
AAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAA
TCTTTATTTCGACGTGTCTACATT
   
Sequence Name 63-K021739-022-232-C03C-8409
GenBank Accession LN510883 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K021739-022-232-C03C-8409
CATTTACATTGAATATATCCCCATCAAACAAACAAAAACAATATTTGACCAAAAATAGAC
AAATGAAGATAAAGGGTTTGATGATATTGGCTTCTTCTCTCCTGATTCTTGCCTTCATTC
ATCAGTCGGAATCAGCTTCAATGCGGAGTTTGCTGATGAATAATGGATCATATGAAGAAG
AAGAACAAGTTCTAAAGTATGACTCCATGGGAACGATCGCTAACTCTTCTGCTTTAGACT
CAAAACGGGTAATTCCCACTGGTCCAAATCCACTCCACAACAGGTAACTGTGGTAATTGA
AGAACAATTATATGTGTATCTATGTTCTTGTGTGTTGTAAGGACATTTCCTAGTTAAGTT
TCATTAATTACTCGATATTCTTTGGCCTTT

Last Updated on 06.12.2018 16:11