Sequences from confirmation amplicon


Confirmation sequences for line 232D11 and hit At3g14410 are shown.
Line ID 232D11
Locus At3g14410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:4817245
Primers used for PCR RK58 - 8474, show primer details
   
Sequence Name 37-K018968-022-232-D11C-RK58
GenBank Accession LN502444 [GenBank]
Sequencing primer gene specific primer, RK58: 5'-GAGCTGGTATGTGCAATGTACTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K018968-022-232-D11C-RK58
AAGTTGACTAGCAGATTTTAGAATCCCTCGGTTGTGTAGAATTCAACATCCGTTTGCTTT
TGTTTGATCGTGTTGTGTTTGCTTTTGACCTCTCTGTTATTGCTTATGCATCAGAGTTCA
CCGTTTTACAATCTGTAGCAAGTAACGATTCATAGATTGTGGTTTCTCTCTTTTCGTCAT
GACTAACGTTTTTGTCTTTGAATTGCTCTGCTGCAGTGGGTTTTGTCATCTAAGGAAATT
AATTTCCCTTATCCTCTTGGATTGACTTTACTCCATATGATCTTTTCCTCTGTCTTGTGC
TTTCTTCTTACCAAAGTTCTTAAGGTAATCTTGCAATTAATGTTCTTTCTGTTTTTGTTT
TTGTGTTTCTGTTATGTAATCATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTAC
ATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTT
TTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGA
TAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTA
ATTCGGAAATCTTTATTTCGACGTGTCTACA
   
Sequence Name 38-K018968-022-232-D11C-8409
GenBank Accession LN502821 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K018968-022-232-D11C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATGATTACATAACAGAAACACAA
AAACAAAAACAGAAAGAACATTAATTGCAAGATTACCTTAAGAACTTTGGTAAGAAGAAA
GCACAAGACAGAGGAAAAGATCATATGGAGTAAAGTCAATCCAAGAGGATAAGGGAAATT
AATTTCCTTAGATGACAAAACCCACTGCAGCAGAGCAATTCAAAGACAAAAACGTTAGTC
ATGACGAAAAGAGAGAAACCACAATCTATGAATCGTTACTTGCTACAGATTGTAAAACGG
TGAACTCTGATGCATAAGCAATAACAGAGAGGTCAAAAGCAAACACAACACGATCAAACA
AAAGCAAACGGATGTTGAATTCTACACAACCGAGGGATTCTAAAATCTGCTAGTCAACTT
CCTAGAAACTAGCACAGTACATTGCACATACCAGC

Last Updated on 06.12.2018 16:11