Sequences from confirmation amplicon


Confirmation sequences for line 232D12 and hit At2g20290 are shown.
Line ID 232D12
Locus At2g20290 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:8751650
Primers used for PCR AS19 - 8474, show primer details
   
Sequence Name 24-K023359-022-232-D12C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K023359-022-232-D12C-8409
CTACACTAACTAATTTTCATTCTCCTTCAGTCCTCCGGAATTTCATTTCTTTATTTCATA
TAGCTCAAATATATGGGACTCACCTTCTGTTCCCTGGTTTTTCCTTTTCTTTCCC
   
Sequence Name 25-K023359-022-232-D12C-AS19
GenBank Accession LN497264 [GenBank]
Sequencing primer gene specific primer, AS19: 5'-AGAAAAACTTCAAAGTTGAATGGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K023359-022-232-D12C-AS19
CACCTGTAAGCTGCATCCGCAACTGCAAACGGATGGGGACTCAATTCTCCCAAAGCGGCT
CCTTTATATTGTGCCATCATATGGTCATTGTAAAGATTCGGTAGTTGTTTAAAAGGGTTT
ACAGCAATCAATATATTTCCTGTGTATGTCTAGTAAGCAAAAAGAAAGCAATTTTTAGGC
CAATCTTACAGAGCAAATTGAAAAAAAAAAAAGAAAAACCAGCGAACAGAAGGAGACTCA
CATATATTTCATCTATATAAAATCTGGATTTCAAATTTTGTAGGACTCCGGGTTCATGTA
AATACGCTAGGGTTGTCATATCACCCACTCCAAAAGGAGGAACTTCCATATCTTTTGGAT
ATGTATTTGAACCTTTGGCAACAACCTGAAAAAAAAATCAACAGAAGTATGAAAAACAAT
CTCATTAAACATATACGGATTCCATCCTTAAGCATCGGGAAAGTATAACTAAATTCGGTT
ATACATACCGTCTTCCCTGAGGTACATTGGACCTTTATATCTTCTCCATTAACCTCAACA
ACTTCACCATCTATCCTTGCCTCCTCTGGATCTTGCACCCAAACAATCGATCAGCAATGA
GTATGATGGTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACTTGGAT

Last Updated on 06.12.2018 16:11