Sequences from confirmation amplicon


Confirmation sequences for line 232H12 and hit At4g27800 are shown.
Line ID 232H12
Locus At4g27800 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:13853268
Primers used for PCR DL82 - 8474, show primer details
   
Sequence Name 07-K018600-022-232-H12C-DL82
GenBank Accession LN489049 [GenBank]
Sequencing primer gene specific primer, DL82: 5'-CTACTTAACTCGCTTTGCCTGACT-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K018600-022-232-H12C-DL82
GTGCTTCTGAGGCCGCATCTTCATCGCTTTCACTCCAACACTCTCCGCCACTCAGCCTAT
CCTTCCGCCGACGCTGGCGGTGGCCTTGTCGTCTATCCCACGTATGGTCGTCACCGTTGC
TCCGCGATTGCGATCGACGCTCCGTCGTCTCTGACTGGCGTGACGCCGATTCGTTGGGGT
TACACGAGTGTTCAAGGATTCCGAGATGAGATGGAAGACGACATCGTAATCCGTTCTGAT
GCTGTCGATAGCTTCTCCTACGCAGCGGTCTTCGACGGCCACGCTGGGTCGTCCTCTGTC
AAATTCCTCAGGTAACTTGTGTTGCTTCTCATGCAAGCTCTGTCTTCTAGGGTTTAAGTG
TTGACGATGGTGATGATGATTGATAGGGAAGAGCTGTATAAAGAGTGTGTTGGTGCATTG
CAAGCTGGATCGTTGTTGAATGGAGGCGATTTCGCAGCAATCAAGGAAGCTTTGATTAAG
GCCTTTGAAAGCGTTGACCGCAATTTGCTCAAATGGTGAAAATGAGCTTAAATTTCTCAG
AGCTTGAATGAATTTTGATGATTTTGAGTGAAATGAAGTGATTCATCATGCGTTTGGATC
CAGGCTAGAAGCTAACGGTGACGAAAAAGATGAATCAGGTTCAACAGCCACTGTAATGAT
CATCAGAAATGATGTTTCCTTCATTGCTCATATTGGTGATTCGTGTGCAGTAAGTCTTCT
TATTTATAAATGGATCATTGTAACTTGGTCATGTCTAAAGTCTTGTTAATGCATTGAAAT
AGGTCTTATCTCGATCTGGGCAAATCGAGGAATTGACTGATATTCAATTGTAAATGGCT
   
Sequence Name 08-K018600-022-232-H12C-8409
GenBank Accession LN489517 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K018600-022-232-H12C-8409
AGATTTCCCGGACATGAAGCCATTTACAATTGAATATCAGTCAATTCCTCGATTTGCCCA
GATCGAGATAAGACCTATTTCAATGCATTAACAAGACTTTAGACATGACCAAGTTACAAT
GATCCATTTATAAATAAGAAGACTTACTGCACACGAATCACCAATATGAGCAATGAAGGA
AACATCATTTCTGATGATCATTACAGTGGCTGTTGAACCTGATTCATCTTCTTCGTCACC
GTTAGCTTCTAGCCTGGATCCAAACGCATGATGAATCACTTCATTTCACTCAAAATCATC
AAAATTCATTCAAGCTCTGAGAAATTTAAGCTCATTTTCACCATTTGAGCAAATTGCGGT
CAACGCTTTCAAAGGCCTTAATCAAAGCTTCCTTGATTGCTGCGAAATCGCCTCCATTCA
ACAACGATCCAGCTTGCAATGCACCAACACACTCTTTATACAGCTCTTCCCTATCAATCA
TCATCACCATCGTCAACACTTAAACCCTAGAAGACAGAGCTTGCATGAGAAGCAACACAA
GTTACCTGAGGAATTTGACAGAGGACGACCCAGCGTGGCCGTCGAAGACCGCTGCGTAGG
AGAAGCTATCGACAGCATCAGAACGGATTACGATGTCGTCTTCCATCTCATCTCGGAATC
CTTGAACACTCGTGTAACCCCAACGAATCGGCGTCACGCCAGTCAGAGACGACGGAGCGT
CGATCGCAATCGCGGAGCAACGGTGACGACCAT

Last Updated on 06.12.2018 16:11