Sequences from confirmation amplicon


Confirmation sequences for line 233B09 and hit At2g36670 are shown.
Line ID 233B09
Locus At2g36670 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:15365408
Primers used for PCR GD05 - 8474, show primer details
   
Sequence Name 62-K100815-0022-233-B09C-8409
GenBank Accession LN510697 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K100815-0022-233-B09C-8409
CCATTTACATATTGACCATCCTGTTAAGATAGGAGAGGTCAAGTAAGAAGAATACATATA
AAAGCAGACTACTTTAATTCTAATAGTTTTATGTACATATAACAAGCAGAAGAACTTACA
TAGATACCGTAATGAAATAAGTAGTCCTGAGGTCTTAACATCATTGATGCACCACCAGCA
AAGTTGAGACTAACTGAAGGAAACATGTCACTTATGCTGCACAAGCACACATAACCATCA
CAACTCTAATCTTAACTTTGATCTAGAGAGAAAAAGGATTTGAACAATGCAGTTATATAT
GAGGAGGCATACCTAGTCGAGACCAAATAGCACTGCTCTCCATTAGAAATTATTGGTGTT
ACCAACTGTGACACGCTTTTTTGATAT
   
Sequence Name 62-K100827-0022-233-B09C-GD05
GenBank Accession LN510699 [GenBank]
Sequencing primer gene specific primer, GD05: 5'-GATATCAAACAGCGTGTCACAGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K100827-0022-233-B09C-GD05
CTATTTGGTCTCGACTAGGTATGCCTCCTCATATATAACTGCATTGTTCAAATCCTTTTT
CTCTCTAGATCAAAGTTAAGATTAGAGTTGTGATGGTTATGTGTGCTTGTGCAGCATAAG
TGACATGTTTCCTTCAGTTAGTCTCAACTTTGCTGGTGGTGCATCAATGATGTTAAGACC
TCAGGACTACTTATTTCATTACGGTATCTATGTAAGTTCTTCTGCTTGTTATATGTACAT
AAAACTATNAGAATTAAAGTAGTCTGCTTTTATATGTATTCTTCTTACTTGACCTCTCCT
ATCTNAACAGGATGGTCAATATGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAGATGTCCGCA

Last Updated on 06.12.2018 16:11