Sequences from confirmation amplicon


Confirmation sequences for line 233B11 and hit At4g22090 are shown.
Line ID 233B11
Locus At4g22090 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:11704728
Primers used for PCR CP78 - 8474, show primer details
   
Sequence Name 54-K105307-0022-233-B11C-CP78
GenBank Accession LN508475 [GenBank]
Sequencing primer gene specific primer, CP78: 5'-TTTTGGTGGTGGAATAATAATGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K105307-0022-233-B11C-CP78
ATATATAAAAAAGGATAAGAAAATTGTAATTAAAGTCTACTTACCTTTCTTGTTTTTGAG
CTAAATTCATTCTTTTTCATAAACAATTTAGGAATTAATCTATAATATGAAATTGGGACC
AAAATGTCCAAAAATACTTTCGGCTAAAAAGACCAACATGACTCTTTTTTTTGTAGGTCA
AAATAGTATTAATAGTCGAGTTTCGAATCTATCTTTTTTTCTTCGTTAAAACTTGCAATA
TGCTGGTTTGTTGAATATATAGATGGTGATACAATTGTAAATGGCTTCATGTCCGGGAAA
TCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACC
AATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGATTATA
   
Sequence Name 38-K105308-0022-233-B11C-8409
GenBank Accession LN502975 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K105308-0022-233-B11C-8409
GCCATTTACAATTGTATCACCATCTATATATTCAACAAACCAGCATATTGCAAGTTTTAA
CGAAGAAAAAAAGATAGATTCGAAACTCGACTATTAATACTATTTTGACCTACAAAAAAA
AGAGTCATGTTGGTCTTTTTAGCCGAAAGTATTTTTGGACATTTTGGTCCCAATTTCATA
TTATAGATTAATTCCTAAATTGTTTATGAAAAAGAATGAATTTAGCTCAAAAACAAGAAA
GGTAAGTAGACTTTAATTACAATTTTCTTATCCTTTTTTATATATTTTAATATCGGTTTC
TAAGGACTGAATAACTGCTTGACCATTATTATTCCACCACCAAA

Last Updated on 06.12.2018 16:11