Sequences from confirmation amplicon


Confirmation sequences for line 233E11 and hit At2g46810 are shown.
Line ID 233E11
Locus At2g46810 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:19240873
Primers used for PCR PH66 - 8474, show primer details
   
Sequence Name 18-K110359-0022-233-E11C-PH66
GenBank Accession LN494352 [GenBank]
Sequencing primer gene specific primer, PH66: 5'-GTAGTGGTTGGTTTCAGAATGGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K110359-0022-233-E11C-PH66
TTTCGAGTTCCCAAGGCTTTTCAGTGTCTGGAGAGATAGTAGTGCTAGAAAGCTTGGGTC
TTTGAATGACAAAAACGGTGAAGGGTCTTCACTTTGTTGCAGCATTTGTAGAAATGGTAT
TGTTGTGTCTTGCAGCAAGCTCGGAGTTGAGAGTTGTTGTTCTTCCTCTTCAAGGGCAAA
GCTTTGATGATCCTCTACAGTTTCTTGAACTTGTAGGCTTCTTCTCACATTCACTTTTTG
GTCCAAACACTGCAACAAAAGAAAAAACAGGGGAAGGAGTTACCTACCAAGTGAGTAAGA
AGAGAAAGTGAGATTGAGAAGAGAGATATTAAATTTGGGTAGACTAGTGTGGGTTTTGGT
TAGTGGGTCAGTCTAGCGAAACAAAATTAGAATGAAAAATGGAATGTTTCAAAGAGAGGG
AAACAGTTGAGAGCAGATTTGTTGGTGTTTTTGGTATAACAAACACATATAGCATTGAAT
GCAACCATTGTGGACTCTTTTGGAAGGAGTAAATGCAAGTCCACATCTTTGAATCAATAG
TTGACCTGACCTGACCACATGGATCAATTGAATCAACATAAACAAAGCTCTGCAGGAGAA
ACATGTTGAGAGTACTACACAACGCCNTCATATGATATTGGATATATTCAATTGTAAATG
GCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAA
AAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATATAGATGTCCGCA
   
Sequence Name 02-K110358-0022-233-E11C-8409
GenBank Accession LN487082 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K110358-0022-233-E11C-8409
TTTACAATTGAATATATCCATATCATATGAAGGCGTTGTGTAGTACTCTCAACATGTTTC
TCCTGCAGAGCTTTGTTTATGTTGATTCAATTGATCCATGTGGTCAGGTCAGGTCAACTA
TTGATTCAAAGATGTGGACTTGCATTTACTCCTTCCAAAAGAGTCCACAATGGTTGCATT
CAATGCTATATGTGTTTGTTATACCAAAAACACCAACAAATCTGCTCTCAACTGTTTCCC
TCTCTTTGAAACATTCCATTTTTCATTCTAATTTTGTTTCGCTAGACTGACCCACTAACC
AAAACCCACACTAGTCTACCCAAATTTAATATCTCTCTTCTCAATCTCACTTTCTCTTCT
TACTCACTTGGTAGGTAACTCCTTCCCCTGTTTTTTCTTTTGTTGCAGTGTTTGGACCAA
AAAGTGAATGTGAGAAGAAGCCTACAAGTTCAAGAAACTGTAGAGGATCATCAAAGCTTT
GCCCTTGAAGAGGAAGAACAACAACTCTCAACTCCGAGCTTGCTGCAAGACACAACAATA
CCATTTCTACAAATGCTGCAACAAAGTGAAGACCCTTCACCGTTTTTGTCATTCAAAGAC
CCAAGCTTTCTAGCACTACTATCTCTCCAGACACTTGAAAAGCCTTGGGAACTCGAAAAC
TACCTCCCACATGAAGTTCCAGAGTTTCATTCACCGATCCCTTCTGAA
Primers used for PCR NH25 - 8474, show primer details
   
Sequence Name 30-K110359-0022-233-E11C-NH25
GenBank Accession LN499767 [GenBank]
Sequencing primer gene specific primer, NH25: 5'-TAGTGCTAGAAAGCTTGGGTCTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K110359-0022-233-E11C-NH25
TTTGTTGCAGCATTTGTAGAAATGGTATTGTTGTGTCTTGCAGCAAGCTCGGAGTTGAGA
GTTGTTGTTCTTCCTCTTCAAGGGCAAAGCTTTGATGATCCTCTACAGTTTCTTGAACTT
GTAGGCTTCTTCTCACATTCACTTTTTGGTCCAAACACTGCAACAAAAGAAAAAACAGGG
GAAGGAGTTACCTACCAAGTGAGTAAGAAGAGAAAGTGAGATTGAGAAGAGAGATATTAA
ATTTGGGTAGACTAGTGTGGGTTTTGGTTAGTGGGTCAGTCTAGCGAAACAAAATTAGAA
TGAAAAATGGAATGTTTCAAAGAGAGGGAAACAGTTGAGAGCAGATTTGTTGGTGTTTTT
GGTATAACAAACACATATAGCATTGAATGCAACCATTGTGGACTCTTTTGGAAGGAGTAA
ATGCAAGTCCACATCTTTGAATCAATAGTTGACCTGACCTGACCACATGGATCAATTGAA
TCAACATAAACAAAGCTCTGCAGGAGAAACATGTTGAGAGTACTACACAACGCCNTCATA
TGATATTGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGC
AATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATT
CAAAAATGTAGATATCCGCA
   
Sequence Name 14-K110358-0022-233-E11C-oriL
GenBank Accession LN492557 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K110358-0022-233-E11C-oriL
ATTTACAATTGAATATATCCAATATCATATGAAGGCGTTGTGTAGTACTCTCAACATGTT
TCTCCTGCAGAGCTTTGTTTATGTTGATTCAATTGATCCATGTGGTCAGGTCAGGTCAAC
TATTGATTCAAAGATGTGGACTTGCATTTACTCCTTCCAAAAGAGTCCACAATGGTTGCA
TTCAATGCTATATGTGTTTGTTATACCAAAAACACCAACAAATCTGCTCTCAACTGTTTC
CCTCTCTTTGAAACATTCCATTTTTCATTCTAATTTTGTTTCGCTAGACTGACCCACTAA
CCAAAACCCACACTAGTCTACCCAAATTTAATATCTCTCTTCTCAATCTCACTTTCTCTT
CTTACTCACTTGGTAGGTAACTCCTTCCCCTGTTTTTTCTTTTGTTGCAGTGTTTGGACC
AAAAAGTGAATGTGAGAAGAAGCCTACAAGTTCAAGAAACTGTAGAGGATCATCAAAGCT
TTGCCCTTGAAGAGGAAGAACAACAACTCTCAACTCCGAGCTTGCTGCAAGACACAACAA
TACCATTTCTACAAATGCTGCAACAAAGTGAAGACCCTTCACCGTTTTTGTCATTCAAAG
ACCCAAGCTTTCTAGCACT

Last Updated on 06.12.2018 16:11