Sequences from confirmation amplicon


Confirmation sequences for line 233F04 and hit At1g14560 are shown.
Line ID 233F04
Locus At1g14560 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:4982201
Primers used for PCR 12cv - 8474, show primer details
   
Sequence Name 32-K109134-0022-233-F04C-12cv
GenBank Accession LN500617 [GenBank]
Sequencing primer gene specific primer, 12cv: 5'-GGTGTTACAATTTGATGGTCCTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K109134-0022-233-F04C-12cv
GTTTGACTCTATTTTGCAGCTTAGCTCTACCACTTTACTAAATTCTTGTGATGTGCATAA
CTCTCGCAGAGGAAATGGGGCGAGTGTTATTCGGATTATTCCATATGCCGCCCTTCATTA
CATGACGTATGAAGTTTATCGTGACTGGATTTTGGAAAAAAATCTTCCCTTAGGTTCTGG
TCCAATTGTTGATCTTGTGGCTGGTTCAGCTGCAGGTGGAACAGCAGTTTTGTGCACATA
TCCCCTTGATTTAGCTCGTACTAAGCTAGCTTACCAGGTTGGTGATTTTGAATACCTTGC
TCTGATAGAATTTTATTGTTGCTTACTTAACTGACAAATGAGCAATATCTTTTTGTTTCT
CCTCTTATGTGTTGTTATTTGCATTTTAGGTTTCTGACACAAGACAGAGTCTCCGAGGTG
GTGCGAATGGATTTTACCGTCAACCTACTTATTCAGGCATAAAAGAGGTGCTTGCAATGG
CTTATAGCCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATC
AGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCA
ATTCAAAAATGTAGATGTCCGCACGGTTATT
   
Sequence Name 16-K109133-0022-233-F04C-8409
GenBank Accession LN493466 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K109133-0022-233-F04C-8409
ATTGAATATATCCTGGCTATAAGCCATTGCAAGCACCTCTTTTATGTCTGAATAAGTAGG
TTGACGGTAAAATCCATTCGCACCACCTCGGAGACTCTGTCTTGTGTCAGAAACCTAAAA
TGCAAATAACAACACATAAGAGGAGAAACAAAAAGATATTGCTCATTTGTCAGTTAAGTA
AGCAACAATAAAATTCTATCAGAGCAAGGTATTCAAAATCACCAACCTGGTAAGCTAGCT
TAGTACGAGCTAAATCAAGGGGATATGTGCACAAAACTGCTGTTCCACCTGCAGCTGAAC
CAGCCACAAGATCAACAATTGGACCAGAACCTAAGGGAAGATTTTTTTCCAAAATCCAGT
CACGATAAACTTCATACGTCATGTAATGAAGGGCGGCATATGGAATAATCCGAATAACAC
TCGCCCCATTTCCTCTGCGAGAGTTATGCACATCACAAGAATTTAGTAAAGTGGTAGAGC
TAAGCTGCAAAATAAGAGTCAAACCACTAGATACTTACTTATAGAATCCGAGAGGACCAT
CAAATTGTAACAC

Last Updated on 06.12.2018 16:11