Sequences from confirmation amplicon


Confirmation sequences for line 234C05 and hit At4g11610 are shown.
Line ID 234C05
Locus At4g11610 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:7014704
Primers used for PCR BJ02 - 8474, show primer details
   
Sequence Name 12-K116292-0022-234-C05C-BJ02
GenBank Accession LN491740 [GenBank]
Sequencing primer gene specific primer, BJ02: 5'-GTATACATGGGAAGTCTTTGACCC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K116292-0022-234-C05C-BJ02
TACGGACAGCTGGTGAAAAGGAAATAGAGATGTGAAGATTGGGAAGATTCTTATAAGGTT
ATCTACTTTAGAGACTGGACGGATCTACACACATTCTTACCCGCTACTTGTTCTTCACCC
TACTGGGGTGAAGAAAATGGGCGAGCTACATATGGCCGTGAGGTTCACATGCATCTCGTT
CGCAAACATGCTTTACCAATACTCAAAACCACTTTTGCCTAAAATGCATTATGTTAGACC
GTTTTCAGTAATGCAGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTA
CATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATT
TTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTATTATAAT
   
Sequence Name 04-K116291-0022-234-C05C-8409
GenBank Accession LN488066 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K116291-0022-234-C05C-8409
CATTTACATTGAATATATCCTGCTGCATTACTGAAAACGGTCTAACATAATGCATTTTAG
GCAAAAGTGGTTTTGAGTATTGGTAAAGCATGTTTGCGAACGAGATGCATGTGAACCTCA
CGGCCATATGTAGCTCGCCCATTTTCTTCACCCCAGTAGGGTGAAGAACAAGTAGCGGGT
AAGAATGTGTGTAGATCCGTCCAGTCTCTAAAGTAGATAACCTTATACGAATCTTCCCAA
TCTTCACATCTCTATTTCCTTTTTCACCTAGCTGTCCGTTATCAAACACACCAACGGTTA
AAACCGTCGCTGGGTCAAAGACTTCCCATGTATACA

Last Updated on 06.12.2018 16:11