Sequences from confirmation amplicon


Confirmation sequences for line 235B04 and hit At1g63380 are shown.
Line ID 235B04
Locus At1g63380 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:23506355
Primers used for PCR GD43 - 8474, show primer details
   
Sequence Name 02-K100850-0022-235-B04C-8409
GenBank Accession LN486951 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K100850-0022-235-B04C-8409
AAGATGCAACAGACTGTGGATCCGGGGCTTACATCTCTTGTTCGCTATCTCATTCACGAT
TCTTCTCAATATGTCACCGGGAATACATACATTGTCGATTCCGGAACTACACTGCCCGGT
GTGCCCATTTTTTCTTCTCTTTCTCCGTAGATTCAATTCTCCATTTCCCGCTTCCTTCTT
CCGCCGCCGGAGCAACCTTCACAACCGCCGTATCAAACCGAATCATCTCCTCGATCGCAA
ACTCCTTAGCGAAATCTTGTAAATACGCCAAAACTTCACCATGACTCGGAAACCTCCTCG
GATCTCTAGATTCCGATACACCGGAACGAACCACGAACGGGAAGTCCCTGTATCCCATAC
ACTCACGGGGGAGATTAGTTCGGAGAGAGCCATAAACGCTCGAATGAACAACGGATCGGG
TCGGGTCAACGCTTAATGGATCGGGTTCGATATGATCGGTGTATATCCAGGTTCCTCCGA
CTTGTTTTTGTCTCTCGAAGACGACCACCGAGTGACCTTCCCGTCGAAGCTCTCGCGCCG
CTACTAATCCGGCGGCTCCGGCGCCGATNACTGCCACGTGGTGTGATCTGATTGGTGATA
AGGAAGGCGCCATTGGTTTTTTTAACGAGATTCGTTAACGATGGTGAATATACTACGGCC
TTTTTATNAATATGTGGCCCAAAAACTCATGATCCACACACACCACGACACAATGTTAAA
AAAACAAAAACAAATTCCTG
   
Sequence Name 02-K100856-0022-235-B04C-GD43
GenBank Accession LN486952 [GenBank]
Sequencing primer gene specific primer, GD43: 5'-GCAGCGTTTAGATAATTTCTCAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K100856-0022-235-B04C-GD43
TGTGGATCATGAGTTTTTGGGCCACATATTTATAAAAAGGCCGTAGTATATTCACCATCG
TTAACGAATCTCGTTAAAAAAACCAATGGCGCCTTCCTTATCACCAATCAGATCACACCA
CGTGGCAGTTATCGGCGCCGGAGCCGCCGGATTAGTAGCGGCGCGAGAGCTTCGACGGGA
AGGTCACTCGGTGGTCGTCTTCGAGAGACAAAAACAAGTCGGAGGAACCTGGATATACAC
CGATCATATCGAACCCGATCCATTAAGCGTTGACCCGACCCGATCCGTTGTTCATTCGAG
CGTTTATGGCTCTCTCCGAACTAATCTCCCCCGTGAGTGTATGGGATACAGGGACTTCCC
GTTCGTGGTTCGTTCCGGTGTATCGGAATCTAGAGATCCGAGGAAGTTTCCGAGTCATGG
TGAAGTTTTGGCGTATTTACCAGATTTCGCTTAGGAGTTTGCGATCGAGGAGATGATTCC
GTTTGATACCGCCGTTGTGAAGGTTGCTCCCGCCGCCGAAGAAGGAAGCGGGAAATGGAG
AATTGAATCTACGGAGAAAGAGAAGAAAAAATGGGCACACCGGGCAGTGT

Last Updated on 06.12.2018 16:11