Sequences from confirmation amplicon


Confirmation sequences for line 235E02 and hit At4g16990 are shown.
Line ID 235E02
Locus At4g16990 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:9559955
Primers used for PCR MK50 - 8474, show primer details
   
Sequence Name 26-K101424-0022-235-E02C-8409
GenBank Accession LN497856 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K101424-0022-235-E02C-8409
TCGGACATGAGCCATTTACAATTGAATACTCATTGCACATCCTATAGCAAATAAGGCCAC
ATGCTATTCTTCTACGTCAACGGCTCATCAACACCACCCAAAATAATATAGGATTATTGT
TGATTTTGCCATTTTGTGACTTAAATCTTTTAGATTTGCCACCTTCAAATATTTTAGATT
TTGCGTTTTCTGCAACTTTAAAAAATGAAACACTTGAGATTTTTTTTTGTAAAATACATA
CTTAATAACAAACACTATACCCTAAAAAAAATATGGAACTAAATAAACCCAAACGTAATG
TGTTAAATTGTATTTTTTAGATATAAAAAATGGCAAAATCTATAAATACCACTAAAAAAA
TCCTTGTCGGGTAATTGGTTCAAAAAAAAATTCAATCCATGTCAAGGAG
   
Sequence Name 66-K101427-0022-235-E02C-MK50
GenBank Accession LN511565 [GenBank]
Sequencing primer gene specific primer, MK50: 5'-CTCCTTGACATGGATTGAATTTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K101427-0022-235-E02C-MK50
ATTTTTTAGTGGTATTATAGATTTTGCCATTTTTTATATCTAAAAAATACAATTTAACAC
ATTACGTTTGGGTTTATTTAGTTCCATATTTTTTTTAGGGTATAGTGTTTGTTATTAAGT
ATGTATTTTACAAAAAAAAATCTCAAGTGTTTCATTTTTTAAAGTTGCAGAAAACGCAAA
ATCTAAAATATTTGAAGGTGGCAAATCTAAAAGATTTAAGTCACAAAATGGCAAAATCAA
CAATAATCCTATATTATTTTGGGTGGTGTTGATGAGCCGTTGACGTAAAAAAATAGCATG
TGGCCTTATTTGCTATAGGATGTGCAATGAGTATTCAATTGTAAATGGCTTCATGTCCGG
GAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAAT
TACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC

Last Updated on 06.12.2018 16:11