Sequences from confirmation amplicon


Confirmation sequences for line 235G01 and hit At1g22360 are shown.
Line ID 235G01
Locus At1g22360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:7896297
Primers used for PCR 23sm - 8474, show primer details
   
Sequence Name 20-K116610-0022-235-G01C-23sm
GenBank Accession LN495374 [GenBank]
Sequencing primer gene specific primer, 23sm: 5'-GTCCAGACACTCAGTCTCCTCTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K116610-0022-235-G01C-23sm
GTCGATTCCTATCTCGCCGCTCTCTTGTTTCTCTAGTAAATGTAACGGTCCAATAGAATA
AACCGGAGGTACAATGGATTTCATAGATTGGATAACGTCGTGTTCGAGATCATCAAACGT
GTTGAGAATGATAGCTGAAGCGCGTTTGGCTCGGTCAGCCTCACGGATGATAAAGTTGAG
CATGATGTCGTCAGGATTAGTCGTTCGGATGAAGCTAGGGATGTCTTTTAGTCTTAGGTT
CTTCATCGATGGTATCCAGTCTATTTTTGTGTCCAAGTGTTCCTTGGTTAAGTAACTCTC
ATCTGCCAAATAAACCACCATATTATTGATCAGAACTCGATATCATCTCAAGATTTCTTA
ACTACAATTAAAGATGCAAATTTGGATGCATTAACATTGATACTGTATACAGGATCAGAG
CCAGCAAAAGGTTATTATAGGTCAAAGATGTCAGACAAAAAGTGGTCTAAATACAATGAT
ATATGGAATGGCTAATTGCATAGAGTTTACTTTTCCAAGTATGTGCTCTCTTTTGGAATT
TAAATTTTATTTTAATTTATCCAGTTTGATTTTATTTTATTTTGCAAGTTATCCATTCAA
TTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAAT
ATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
AGCGTTATTAT
   
Sequence Name 04-K116609-0022-235-G01C-8409
GenBank Accession LN488075 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K116609-0022-235-G01C-8409
ATTTACATTGAATGGATAACTTGCAAAATAAAATAAAATCAAACTGGATAAATTAAAATA
AAATTTAAATTCCAAAAGAGAGCACATACTTGGAAAAGTAAACTCTATGCAATTAGCCAT
TCCATATATCATTGTATTTAGACCACTTTTTGTCTGACATCTTTGACCTATAATAACCTT
TTGCTGGCTCTGATCCTGTATACAGTATCAATGTTAATGCATCCAAATTTGCATCTTTAA
TTGTAGTTAAGAAATCTTGAGATGATATCGAGTTCTGATCAATAATATGGTGGTTTATTT
GGCAGATGAGAGTTACTTAACCAAGGAACACTTGGACACAAAAATAGACTGGATACCATC
GATGAAGAACCTAAGACTAAAAGACATCCCTAGCTTCATCCGAACGACTAATCCTGACGA
CATCATGCTCAACTTTATCATCCGTGAGGCTGACCGAGCCAAACGCGCTTCAGCTATCAT
TCTCAACACGTTTGATGATCTCGAACACGACGTTATCCAATCTATGAAATCCATTGTACC
TCCGGTTTATTCTATTGGACCGTTACATTTACTAGAGAAACAAGAGAGCGGCGAGTATAG
TGAAATCGGACGGACAGGATCGAATCTTTGGAGAGAGGAGACTGATGNTCTGGAC

Last Updated on 06.12.2018 16:11