Sequences from confirmation amplicon


Confirmation sequences for line 236B06 and hit At3g21390 are shown.
Line ID 236B06
Locus At3g21390 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:7534330
Primers used for PCR 07qw - 8474, show primer details
   
Sequence Name 37-K115837-0022-236-B06C-07qw
GenBank Accession LN502737 [GenBank]
Sequencing primer gene specific primer, 07qw: 5'-TTCTAGCTTTCAGCTTTTCCTTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K115837-0022-236-B06C-07qw
TCTGTCATCCGCTTGATGTTGTCAGAAAAGATTCCAGGTTAGTTTTTTGCCATCTTGTGT
TTTAGCTATGTGTTCTTAATGTTTTACCACCGAAGTCCATGTTACCAGAAGCCTAAAGTG
ATTTTACAAAGCATTTTAGATCAAACATCAAGGTTTGATACAAGAATGTTTGTCGCTGCA
GGTCGAAGGTCTGCAACGTCACCCAAAATATGGAGCCCGAGTAGAGCTGAATGCTTACAA
GAACATGTTTGACGGTTTAGGGCAGATATTAAGATCTGAAGGTTGGCACGGGCTTTATAA
AGGCATTGTTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAGATGTCCGCAC
   
Sequence Name 05-K115833-0022-236-B06C-8409
GenBank Accession LN488489 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K115833-0022-236-B06C-8409
TGCCTTTATAAAGCCCGTGCCAACCTTCAGATCTTAATATCTGCCCTAAACCGTCAAACA
TGTTCTTGTAAGCATTCAGCTCTACTCGGGCTCCATATTTTGGGTGACGTTGCAGACCTT
CGACCTGCAGCGACAAACATTCTTGTATCAAACCTTGATGTTTGATCTAAAATGCTTTGT
AAAATCACTTTAGGCTTCTGGTAACATGGACTTCGGTGGTAAAACATTAAGAACACATAG
CTAAAACACAAGATGGCAAAAAACTAACCTGGAATCTTTTCTTGACAACATCAAGCGGAT
GACAGACTAGTTTAGAAACAGTGCCCGAAGCTAAACCGCANAGGAAAAGCTAAAAAGCTA
GA

Last Updated on 06.12.2018 16:11