Sequences from confirmation amplicon


Confirmation sequences for line 236D02 and hit At1g52450 are shown.
Line ID 236D02
Locus At1g52450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:19542155
Primers used for PCR MR78 - 8474, show primer details
   
Sequence Name 11-K017453-022-236-D02C-MR78
GenBank Accession LN490898 [GenBank]
Sequencing primer gene specific primer, MR78: 5'-GATGGAAAATTCTTTTTGTTTGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K017453-022-236-D02C-MR78
AATTAGTCATGTACATATGATAAGGACTCTCTGTATATGAACAAATGTAGTTTCCAAGAC
AAAATATTATAGAATAGAGAGCACAACATCTTTTGAATTGGAGCATGTCACTGTTTTATT
TACATCAATGCCGGGATCCTCTATATTGATACACTTGTTAGGCCATCGATCCGACAGCTT
CATAAAATAGAATCTCTGGTCGAATCTTCCTTTCTCCAAAGAAACTGACCACACTCTTCC
AGTTGCCATCATCCTATAGCATCACCACATATGTGTCAATAATGTCTAGCCTATCTAATT
TCACAATTCAACTCAAGGAAGACAAAGGGAAACTGTTTCGTCTTACCTCTCCTGATAAAG
ACTCTTCTCTGAGATTGACCTTAAAATCTCCAAAGAAAATGACCGCACTCTTCCAGTGAC
TGACATCCTATAGCATCACCACATATGTGTCAATAATGTCTGGCATATCCTAATTCACAG
TTCAACTCAAGCAAGACAAAGGGAAACTGTTTCTTCTTACCTCTCCTGCTAAAGACTCTC
GTCCCAGATTGACCCACCGGTTGTTTTCATAAGCTAAACAAATGTGTTCTTCTTCTTCAC
CACAACCAACCTGCATCACATACGTTGAGTAAATGAGTATCAACCTGCATCACCGGTTGT
TTTATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATG
ATGGTCAATAT
   
Sequence Name 12-K017453-022-236-D02C-8409
GenBank Accession LN491355 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K017453-022-236-D02C-8409
CATGTAGATTTCCCGGNCATGAACCATCTTCATTGAATAAAACAACCGGTGATGCAGGTT
GATACTCATTTACTCAACGTATGTGATGCAGGTTGGTTGTGGTGAAGAAGAAGAACACAT
TTGTTTAGCTTATGAAAACAACCGGTGGGTCAATCTGGGACGAGAGTCTTTAGCAGGAGA
GGTAAGAAGAAACAGTTTCCCTTTGTCTTGCTTGAGTTGAACTGTGAATTAGGATATGCC
AGACATTATTGACACATATGTGGTGATGCTATAGGATGTCAGTCACTGGAAGAGTGCGGT
CATTTTCTTTGGAGATTTTAAGGTCAATCTCAGAGAAGAGTCTTTATCAGGAGAGGTAAG
ACGAAACAGTTTCCCTTTGTCTTCCTTGAGTTGAATTGTGAAATTAGATAGGCTAGACAT
TATTGACACATATGTGGTGATGCTATAGGATGATGGCAACTGGAAGAGTGTGGTCAGTTT
CTTTGGAGAAAGGAAGATTCGACCAGAGATTCTATTTTATGAAGCTGTCGGATCGATGGC
CTAACAAGTGTATCAATATAGAGGATCCCGGCATTGATGTAAATAAAACAGTGACATGCT
CCCCATTCAAAGATGTTGTGCTCTCTATTCTATAATATTTTGTCTTGGAAACTACATTTG
TTCATATACAGAGAGTCCTTATCATATATACATGACTAATTAGTGAG

Last Updated on 06.12.2018 16:11