Sequences from confirmation amplicon


Confirmation sequences for line 236G04 and hit At5g39170 are shown.
Line ID 236G04
Locus At5g39170 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:15681137
Primers used for PCR 18hm - 8474, show primer details
   
Sequence Name 03-K112913-0022-236-G04C-18hm
GenBank Accession LN487562 [GenBank]
Sequencing primer gene specific primer, 18hm: 5'-ACACTATACAGTTGCCTAGGTCCC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K112913-0022-236-G04C-18hm
AAATTTTGTTTTAGACGATGCATAGATTGGACTACGTTAGTAATATACAACTACAAATAA
TTTATTTAAGACCTAAAATAATTGAAAGAAAAAACCAAAAATGAGGTACCACTTGTAGGA
GTATGTAATCTTAACTAGTATGAACAATGAGAAGTCAGACACTCAGACCTTAAATAATTG
AAAGACAAAGCCAATAATGAGGTATCACTTGTGGGAGTATGTCATCTTTACTAGTATGAA
CAATGAGATATATATATAATCGCAGAAAAACATATTGGTGTTATTTTCGTTAGCGACTGC
TCCGATCTACTAAGATGGTGTATGTGAATGTCGGATTCGCACACTCAAGTATTTATTTAT
TTTGTGAGAAAGATTATTTTTGAAGACAAAAATTAAATGTAAAAGCTAGAGATTAACACT
CAAATGACTATNGTATGATTTAGAAAAATTGTGTGTGTGTTAGATAGTCCCAAACCTCTA
CTACTTATACTAAAACATCGTATTTACATGGGAGACCATAGTTTCTCCAAGATGTATAAA
TTTGGAGAACTCACCAATTTCGAATATACTGCTTCGCCCTATATTAGCTACTATAACAAT
AACATTGATNACAAAATTGCTCCGACTGACGCTGGAACCAAAGAAGTCAATATAACCATG
GTGGAAACCGGACCAAAAACCGGTNATCGAGATGGCTTAACCAATGTTCCTAAACCGGAT
ATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGA
TGGTCAATATGGAGAANAAGAAAGAGTAATTACCAATTTTTTTTCAATTCANAAATGTAG
ATGTCCG
   
Sequence Name 01-K112912-0022-236-G04C-8409
GenBank Accession LN486648 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K112912-0022-236-G04C-8409
GCATTTCATTGAATAATCCGGTTTAGGAACTTGGTTAAGCCATCTCGATTACCGGTTTTT
GGTCCGGTTTCCACCATGGTTATATTGACTTCTTTGGTTCCAGCGTCAGTCGGAGCAATT
TTGTTATCAATGTTATTGTTATAGTAGCTAATATAGGGCGAAGCAGTATATTCGAAATTG
GTGAGTTCTCCAAATTTATACATCTTGGAGAAACTATGGTCTCCCATGTAAATACGATGT
TTTAGTATAAGTAGTAGAGGTTTGGGACTATCTAACACACACACAATTTTTCTAAATCAT
ACTATAGTCATTTGAGTGTTAATCTCTAGCTTTTACATTTAATTTTTGTCTTCAAAAATA
ATCTTTCTCACAAAATAAATAAATACTTGAGTGTGCGAATCCGACATTCACATACACCAT
CTTAGTAGATCGGAGCAGTCGCTAACGAAAATAACACCAATATGTTTTTCTGCGATTATA
TATATATCTCATTGTTCATACTAGTAAAGATGACATACTCCCACAAGTGATACCTCATTA
TTGGCTTTGTCTTTCAATTATTTAAGGTCTGAGTGTCTGACTTCTCATTGTTCATACTAG
TTAAGATTACATACTCCTACNAGTGGTACCTCATTTTTGGTTTTTTCTTTCAATTATNTT
AGGTCTTAAATAAATTATTTGTAGTTGTATATTACTAACGTAGTCCAATCTATGCATCGT
CTAANACAAAATTTATGTAATTTTAGACGATNAANGCGGTCATCTATGCATCGTCTAAAC
GTCTAATCGGGACCTAGGC
Primers used for PCR 01jc - 8474, show primer details
   
Sequence Name 04-K112913-0022-236-G04C-01jc
GenBank Accession LN487992 [GenBank]
Sequencing primer gene specific primer, 01jc: 5'-CCATAGTTTCTCCAAGATGTATAAATT-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K112913-0022-236-G04C-01jc
ATTTGATTCTGCTTCCCCTATATTAGCTACTATAACAATAACATTGATAACAAAATTGCT
CCGACTGACGCTGGAACCAAAGAAGTCAATATAACCATGGTGGAAACCGGACCAAAAACC
GGTAATCGAGATGGCTTAACCAATGTTCCTAAACCGGATATATTCAATTGTAAATGGCTT
CATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACCGTTATTA
   
Sequence Name 02-K112912-0022-236-G04C-oriL
GenBank Accession LN487109 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K112912-0022-236-G04C-oriL
GCATTTCATTGAATATATCCGGTTTAGGAACATTGGTTAAGCCATCTCGATTACCGGTTT
TTGGTCCGGTTTCCACCATGGTTATATTGACTTCTTTGGTTCCAGCGTCAGTCGGAGCAA
TTTTGTTATCAATGTTATTGTTATAGTAGCTAATATAGGGCGAAGCAGTATATTCGAAAT
TGGTGAGTTCTCCAAATTTATACATCTTGGAGAAACTATGG

Last Updated on 06.12.2018 16:11