Sequences from confirmation amplicon


Confirmation sequences for line 236G11 and hit At4g04970 are shown.
Line ID 236G11
Locus At4g04970 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:2540653
Primers used for PCR VL37 - 8474, show primer details
   
Sequence Name 05-K024206-022-236-G11C-VL37
GenBank Accession LN488185 [GenBank]
Sequencing primer gene specific primer, VL37: 5'-GAAATTAGCAGAATGGCTAGTGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K024206-022-236-G11C-VL37
AGGGTCTGAGTATGGGAGTGCAATGATGAAATTCACATATGTTGTGGCATGCCAAGTTTA
TGGGCAACATAAAGCAAGAGGGGACCATAGAGCTGAGGAGATTCTGTTCCTGATGAAGAA
TCATGACGCCCTTCGTATTGCCAGGAGTTGATGAGGTTGACCTGGGAAGGGGTGAGGTCG
AGTACTATTCAGTTCTTGTGAAATTTGATCAACAACTTCAGAGAGAGGTGGAGATCTATC
GTATCAGACTGCCAGGCCCCTTGAAGCTTGGTGAGGGAAAACCAGAAAACCAAAACCATG
CTCTAATCTTTACACGAGGAGATGCGATTCAGACCATTGATATGAATCAAGATAACCATT
TTGAGGAGGGGACCATAGTATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGG
ATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTT
CAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAA
CGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCG
GAAATCTTTATTTCGACGTGT
   
Sequence Name 06-K024206-022-236-G11C-8409
GenBank Accession LN488634 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K024206-022-236-G11C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGAATACTATGGTCCCCTCCTCAAAATG
GTTATCTTGATTCATATCAATGGTCTGAATCGCATCTCCTCGTGTAAAGATTAGAGCATG
GTTTTGGTTTTCTGGTTTTCCCTCACCAAGCTTCAAGGGGCCTGGCAGTCTGATACGATA
GATCTCCACCTCTCTCTGAAGTTGTTGATCAAATTTCACAAGAACTGAATAGTACTCGAC
CTCACCCCTTCCCAGGTCAACCTCATCAACTCCTGGCAATACGAAGGGCGTCATGATTCT
TCATCAGGAACAGAATCTCCTCAGCTCTATGGTCCCCTCTTGCTTTATGTTGCCCATAAA
CTTGGCATGCCACAACATATGTGAATTTCATCATTGCACTCCCATACTCAGACCCTTTAA
GCAAGTGGGTTATACCACTAGCCATTCT

Last Updated on 06.12.2018 16:11