Sequences from confirmation amplicon


Confirmation sequences for line 237A08 and hit At3g57080 are shown.
Line ID 237A08
Locus At3g57080 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:21127321
Primers used for PCR PB31 - 8474, show primer details
   
Sequence Name 36-K102502-0022-237-A08C-8409
GenBank Accession LN502175 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K102502-0022-237-A08C-8409
ATTTACAATTGAAAGAATGGATAATGGACCTGTTTTTATTTATTACGGTTCAAAAAGTGA
GACTATTACTTTTATTTAGCTGCAAGAAATGGATAATGGACCTGTTTTTCCTCAATGCTG
AACTTCTTGAGAAGTGTTGTCTTCTCTTCATCATTCAACACCTGATGTTGTGGCTTCAGT
GAATGTTTGGTTATGTTGACTAGCAGATCCGTTATCTTAACAAGACAGCAAAAAGGAAAC
TAGATGTTACAGATTTGAAGTACTAACCAAGAAAAATTAGATTAGGAAAATATATAGGTG
TACCAACCTGGAATATCTCGACCTTGAAAGAAAAAAGTTCAATGGCTTTCAAGGCTTGAT
TGGTTACATGGTTTTGCAGAACCAATATCAGCCCAGTTATGGTTTCTTGGCTAAGTATAT
CCGCTACTACACTGCGGATTGCGTTGACTTTAACCATACTAGTACCAAAAAAGACAATTT
TTACCTGAACAAGCAAAATCACAACACTTCTTGAAGCAGACCCTTTAAACCAATAACACA
ATGTTACTATATCCAGAGAATCACTATGAGATATCTGTCGTTATGCCATAAGACTAGAGC
TGATGCAAGAAACAT
   
Sequence Name 36-K102503-0022-237-A08C-PB31
GenBank Accession LN502176 [GenBank]
Sequencing primer gene specific primer, PB31: 5'-TTTTCAACTCATGATGTTTCTTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K102503-0022-237-A08C-PB31
ACGACGATATCTCATAGTGATTCTCTGGATATAGTAACATTGTGTTATTGGTTTAAAGGG
TCTGCTTCAAGAAGTGTTGTGATTTTGCTTGTTCAGGTAAAAATTGTCTTTTTTGGTACT
AGTATGGTTAAAGTCAACGCAATCCGCAGTGTAGTAGCGGATATACTTAGCCAAGAAACC
ATAACTGGGCTGATATTGGTTCTGCAAAACCATGTAACCAATCAAGCCTTGAAAGCCATT
GAACTTTTTTCTTTCAAGGTCGAGATATTCCAGGTTGGTACACCTATATATTTTCCTAAT
CTAATTTTTCTTGGTTAGTACTTCAAATCTGTAACATCTAGTTTCCTTTTTGCTGTCTTG
TTAAGATAACGGATCTGCTAGTCAACATAACCAAACATTCACTGAAGCCACAACATCAGG
TGTTGAATGATGAAGAGAAGACAACACTTCTCAAGAAGTTCAGCATTGAGGAAAAACAGG
TCCATTATCCATTTCTTGCAGCTAAATAAAAGTAATAGTCTCACTTTTTGAACCGTAATA
AATAAAAACAGGTCCATTATCCATTTCTTTCAATTGTAAATGGCTTCATGTCCGGGAAAT
CTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCA
ATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATT
TTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTAT
TCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTC

Last Updated on 06.12.2018 16:11