Sequences from confirmation amplicon


Confirmation sequences for line 237C07 and hit At1g74480 are shown.
Line ID 237C07
Locus At1g74480 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:27993558
Primers used for PCR FQ70 - 8474, show primer details
   
Sequence Name 83-K033881-022-237-C07C-FQ70
GenBank Accession LN514261 [GenBank]
Sequencing primer gene specific primer, FQ70: 5'-CAATCCCCAATTTCTCACTAAAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>83-K033881-022-237-C07C-FQ70
AAAACCACTAGTAAATTCACTTGAGAAACACACAAGAGATACTTCATTTCTTCATCACTC
TCCATTCCTGCTTCATCAACCGACTCATCACTAGGAACTGATCCGCTGCTTGAACACGAG
GGTACTTGAGACTGATCGGACTTGAGACTTCTCTTCTTCTTCCTCTTGTGGTTAGCCTTG
AAACAAGCTTGTCTTAGCCTCTTTGTCTTGTCCTTAAACTCCAAATCCGGCAAATCCTCA
ATTGTCCTCTTCTCCTTCTCAAGCATCTCCAACGCGTCCTGCAGTTTTTCTGCATTCTCT
TCGCCTTCCATCTTCTGCAGCTCCTATTCATATGATAAAACATTTGAGTTCAATTATCTG
AAAACAATAATATTAAGTGTAGTGCCTTGTTCTACATACCTTGACGTTACTGATCAAAGT
GTTTAAGCTCATAAGTTTACGATGAGGCCATCGGCGAATACCCAATTCGCGACATCTCCT
TTTTAGTAGAGTTAAACCAACGTTAAGTGCTATTGCAGCCTGAAATAGACAGGATATATT
CAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTC
AATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
CGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGT
ATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGT
CTACATT
   
Sequence Name 27-K033881-022-237-C07C-8409
GenBank Accession LN498284 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K033881-022-237-C07C-8409
GAAGCCATTTACAATTGAATATATCCTGTCTATTTCAGGCTGCAATAGCACTTAACGTTG
GTTTAACTCTACTAAAAAGGAGATGTCGCGAATTGGGTATTCGCCGATGGCCTCATCGTA
AACTTATGAGCTTAAACACTTTGATCAGTAACGTCAAGGTATGTAGAACAAGGCACTACA
CTTAATATTATTGTTTTCAGATAATTGAACTCAAATGTTTTATCATATGAATAGGAGCTG
CAGAAGATGGAAGGCGAAGAGAATGCAGAAAAACTGCAGGACGCGTTGGAGATGCTTGAG
AAGGAGAAGAGGACAATTGAGGATTTGCCGGATTTGGAGTTTAAGGACAAGACAAAGAGG
CTAAGACAAGCTTGTTTCAAGGCTAACCACAAGAGGAAGAAGAAGAGAAGTCTCAAGTCC
GATCAGTCTCAAGTACCCTCGTGTTCAAGCAGCGGATCAGTTCCTAGTGATGAGTCGGTT
GATGAAGCAGGAATGGAGAGTGATGAAGAAATGAAGTATCTCTTGTGTGGTTTCTCAAGT
GAATTTACTAGTGGTTTGTGAAATCTGTTTTTAGTGAGAAATGGGGGGATTGAAAAAAAA
ACTTTTAGGTCAGTACTGAAACTATTTAAATTTGCAAATAAAATATATAAAATAAAACCA
AAACTTGCATATAGGGAATCTTTTTTAAAAAAAAAAAAA

Last Updated on 06.12.2018 16:11