Sequences from confirmation amplicon


Confirmation sequences for line 237H07 and hit At3g55640 are shown.
Line ID 237H07
Locus At3g55640 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:20641826
Primers used for PCR 07be - 8474, show primer details
   
Sequence Name 05-K107367-0022-237-H07C-07be
GenBank Accession LN488380 [GenBank]
Sequencing primer gene specific primer, 07be: 5'-TTGCCATTAGCTTTTCTGTGTATG-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K107367-0022-237-H07C-07be
TCACTAGGTAATAGTCGATCTCGATATTACAGTTTTGCAGTTTAACTATGCCACTTATCA
TTTATAATTACTCCTAAAATGATTTGTTGGTTTTCATTTTCTTAAGGCCCCATGATTCCC
CTATCATGGTCAGTCTAGCTTGTGGAAGTCTTTCAGGAATAGCATCTTCAACAGGTAAGT
TATCTTAGCTATTCGTTGGCCTTTTGCTTTCTTGTTTGCCATGAGACTCACTGTTTGACT
GTAATTTGTCATTCTATGCCTTGCTCATAAACACTGATTGACTTAGCATGAGCATCAATC
AGTTTCGTATTGCTGACAAGCATCACATCAATAGTCTCAAACTCAAAATTCTCTGTACTA
TGCTTTCAATTCACAATAGCTGAACATGGTGCATTCTAAGTTCCACTTTTGCTACAGAAT
GATAAATCTGTTGCTCCTTCTCATATGGATATTCAATGGCTTCATGTCCGGGAAATCTAC
ATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTT
TTTTTCAATTCAAAAATGTAGATGTCCGCACGNTTATTA
   
Sequence Name 21-K107368-0022-237-H07C-8409
GenBank Accession LN495687 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K107368-0022-237-H07C-8409
GCCTTGATATCCATATGAGAAGGAGCAACAGATTTATCATTCTGTAGCAAAAGTGGAACT
TAGAATGCACCATGTTCAGCTATTGTGAATTGAAAGCATAGTACAGAGAATTTTGAGTTT
GAGACTATTGATGTGATGCTTGTCAGCAATACGAAACTGATTGATGCTCATGCTAAGTCA
ATCAGTGTTTATGAGCAAGGCATAGAATGACAAATTACAGTCAAACAGTGAGTCTCATGG
CAAACAAGAAAGCAAAAGGCCAACGAATAGCTAAGATAACTTACCTGTTGAAGATGCTAT
TCCTGAAAGACTTCCACAAGCTAGACTGACCATGATAGGGGAATCATGGGGCCTTAAGAA
AATGAAAACCAACAAATCATTTTAGGAGTAATTATAAATGATAAGTGGCATAGTTAAACT
GCAAAACTGTAATATCGAGATCGACTTATTACCTAGTTGACCTCCAATAAGATCTCAATG
ATTCATACACAGAAAAGCAAATGGCA

Last Updated on 06.12.2018 16:11