Sequences from confirmation amplicon


Confirmation sequences for line 238A06 and hit At3g53920 are shown.
Line ID 238A06
Locus At3g53920 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:19961847
Primers used for PCR GI01 - 8474, show primer details
   
Sequence Name 02-K018753-022-238-A06C-GI01
GenBank Accession LN486837 [GenBank]
Sequencing primer gene specific primer, GI01: 5'-CAAAACTGAACCAAACTGAACAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K018753-022-238-A06C-GI01
ACTGAACCAATCTTTATGATAAACCTGATGGTAACAGTATTTTCATATCCAAAAAACCAA
ATTTAAACTTAAACTAATCAAATCTTGGTCCTTGTTCGATAGACATTTCTCAAAACCAAA
GATTGAAGCAAACTAAACAGACATACCAGGAATTTTGTAGTAAAGCAATCCCCAACTTTC
TTGTCAATTGAACCGACCACTTTTAGGCATTGGTTAGCTGCTCGAATCTTCTTCACCGAG
TGGCCTGTTAGTTTTGCAATCTCCTCATCAGCCGCATATTTTATACCATGGCTCGTTTTC
AGGGTCTTACGAGCCTTTTGTATATGATTGATAGTTCGGATTATTGATGACTGCCAAAAA
AACAGAAACAATAAAGGAAAACAAGTGAGGTTCTAGCTTTGTTGAAAATGAGACAAAAAG
GGCCAAAAATCAAAATGCACAAACAGGAATATATTCAATTGTAAATGGCTTCATGTCCGG
GAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAAT
TACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGT
ACTTTTTGATAAAACGACAAATTAC
   
Sequence Name 06-K018753-022-238-A06C-8409
GenBank Accession LN488604 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K018753-022-238-A06C-8409
CATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATTCCTGTTTGTGCATTT
TGATTTTTGGCCCTTTTTGTCTCATTTTCAACAAAGCTAGAACCTCACTTGTTTTCCTTT
ATTGTTTCTGTTTTTTTGGCAGTCATCAATAATCCGAACTATCAATCATATACAAAAGGC
TCGTAAGACCCTGAAAACGAGCCATGGTATAAAATATGCGGCTGATGAGGAGATTGCAAA
ACTAACAGGCCACTCGGTGAAGAAGATTCGAGCAGCTAACCAATGCCTAAAAGTGGTCGG
TTCAATTGACAAGAAAGTTGGGGATTGCTTTACTACAAAATTCCTGGTATGTCTGTTTAG
TTTGCTTCAATCTTTGGTTTTGAGAAATGTCTATCGAACAAGGACCAAGATTTGATTAGT
TTAAGTTTAAATTTGGTTTTTTGGATATGAAAATACTGTTACCATCAGGTTTATCATAAA
GATTGGTTCAGTTTAGATTTAAGCTATTCTTTTTGTTCAGTTTGG

Last Updated on 06.12.2018 16:11