Sequences from confirmation amplicon


Confirmation sequences for line 238H10 and hit At1g49760 are shown.
Line ID 238H10
Locus At1g49760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:18418597
Primers used for PCR 42kf - 8474, show primer details
   
Sequence Name 28-K113872-0022-238-H10C-42kf
GenBank Accession LN498933 [GenBank]
Sequencing primer gene specific primer, 42kf: 5'-CTCTAAATGGAAAGACCTTTGACG-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K113872-0022-238-H10C-42kf
GAGTCCGAGAGGGAACTGAACTCAACAAAAGTTTGAGCAAAGTTTGAAGGAGGCTGCAGA
CAAGTCTCAGGGGTCGAACTTATATGTTAAGAACTTGGATGAGAGCGTCACAGATGATAA
GCTCAGAGAACATTTTGCTCCCTTTGGAACTATTACATCATGCAAGGTCCGTGACTGATA
CAATAAAAAGCGCTGCTTGTTACTATCTTCAGCAGATTTGCATTAAAATGTATTACATTT
GAACGTGACAGGTGATGCGTGACCCCAGTGGAGTGAGTAGAGGATCTGGATTTGTCGCAT
TTTCAACTCCTGAGGAAGCAACTAGAGCTGTAAGTGACTAGAATATAAAGAAGACATGTC
AAATTTTGGTGATCTCCTATTCTCTGGAAGGATCTTGATGCTTAACACTGTCTTCTTTTC
AATGTTTCAGATTACAGAGATGAATGGAAAAATGATCGTTACCAAACCTCTATATGTTGC
TCTTGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGC
AATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATT
CAAAAATGTAGATGTCCGCA
   
Sequence Name 02-K113871-0022-238-H10C-8409
GenBank Accession LN487116 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K113871-0022-238-H10C-8409
TCGGACTGAGCCTTTCATTGAATATATCCTGCAAGAGCAACATATAGAGGTTTGGTAACG
ATCATTTTTCCATTCATCTCTGTAATCTGAAACATTGAAAAGAAGACAGTGTTAAGCATC
AAGATCCTTCCAGAGAATAGGAGATCACCAAAATTTGACATGTCTTCTTTATATTCTAGT
CACTTACAGCTCTAGTTGCTTCCTCAGGAGTTGAAAATGCGACAAATCCAGATCCTCTAC
TCACTCCACTGGGGTCACGCATCACCTGTCACGTTCAAATGTAATACATTTTAATGCAAA
TCTGCTGAAGATAGTAACAAGCAGCGCTTTTTATTGTATCAGTCACGGACCTTGCATGAT
GTAATAGTTCCAAAGGGAGCAAAATGTTCTCTGAGCTTATCATCTGTGACGCTCTCATCC
AAGTTCTTAACATATAAGTTCGACCCCTGAGACTTGTCTGCAGCCTCCTTCAAACTTTGC
TCAAACTTTTGTTTGAGTTCAGTTTCCCTCTCGGACTTCTTTTGGGCCTTCCCAACAAAC
CACTCCTTGTCGTCAAAGGTCTTTCANTTTTAGA

Last Updated on 06.12.2018 16:11