Sequences from confirmation amplicon


Confirmation sequences for line 241B06 and hit At5g19530 are shown.
Line ID 241B06
Locus At5g19530 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:6589149
Primers used for PCR MR79 - 8474, show primer details
   
Sequence Name 15-K017452-022-241-B06C-MR79
GenBank Accession LN492748 [GenBank]
Sequencing primer gene specific primer, MR79: 5'-GTAAGCAAATTAAAGCCTGTTTCG-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K017452-022-241-B06C-MR79
GTGTCTTGTATGCATTATTGGATCCTCTTTTTTGATGATTGATATAAAAACAGACGTGAA
GGCTTACACAGCACATGTGCCATCATTTGCGGACACATGGGGATGGGTGATGGCATCGGA
CCACGAGTTTGACGTTGAAGTTGATGAAATGGATCGAAGAATCGAAGAGAGAGTTAACGG
AGAATTGATGTATCTAAACGCTCCTTCTTTCGTCTCTGCTGCTACTCTCAACAAAACCAT
CTCTCTCGCGTAATTTCCATTTTTCTCTCTTTTATTTTCTTTTCTTAACACAATAAATAA
TTATTCGATTTATTTACAAAATTTTTGAAATTAAAAAATTGAGCAGGCTAGAGAAGGAGA
CTGAAGTTTATAGTGAAGAGAATGCGAGATTCATTCATGGTCATGGTGTGGCGTACCGGC
ATATTTAAAGACGAACCGGTTTCAGTTTCAGTGTTTGGTGCTGGCAGGATATATTCAATT
GTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATAT
GGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAG
CGTTATTATAAAATGANAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTA
TAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCT
   
Sequence Name 16-K017452-022-241-B06C-8409
GenBank Accession LN493214 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K017452-022-241-B06C-8409
ATTTCCCGGAATGAAGCCACTTACAATTGAATATATCCTGCCAGCACCAAACACTGAAAC
TGAAACCGGTTCGTCTTTAAATATGCCGGTACGCCACACCATGACCATGAATGAATCTCG
CATTCTCTTCACTATAAACTTCAGTCTCCTTCTCTAGCCTGCTCAATTTTTTAATTTCAA
AAATTTTGTAAATAAATCGAATAATTATTTATTGTGTTAAGAAAAGAAAATAAAAGAGAG
AAAAATGGAAATTACGCGAGAGAGATGGTTTTGTTGAGAGTAGCAGCAGAGACGAAAGAA
GGAGCGTTTAGATACATCAATTCTCCGTTAACTCTCTCTTCGATTCTTCGATCCATTTCA
TCAACTTCAACGTCAAACTCGTGGTCCGATGCCATCACCCATCCCCATGTGTCCGCAAAT
GATGGCACATGTGCTGTGTAAGCCTTCACGTCTGTTTTTATATCAATCATCAAAAAATGG
ATCCAATAATGTCATTACAAGAACACACACGAAACAGGCTTTAAATTTGCTT

Last Updated on 06.12.2018 16:11