Sequences from confirmation amplicon


Confirmation sequences for line 241D05 and hit At4g09820 are shown.
Line ID 241D05
Locus At4g09820 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:6183022
Primers used for PCR MH11 - 8474, show primer details
   
Sequence Name 57-K022449-022-241-D05C-MH11
GenBank Accession LN509284 [GenBank]
Sequencing primer gene specific primer, MH11: 5'-TCTGGGTAAACAACTCTCTCCCTA-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K022449-022-241-D05C-MH11
GTTTCTAAATTTGTTCTTTCTTTTTTTTCCTTCTCTAAGTGAAGACAAAAATAGTATTGT
GTGTTAAATGCGAATATCGAAAATATTGTGTTGACATTAAAGAGAAACTGAGGAAGTTGA
GTTACTAGAATAAGAAAGAAATGAGAGTTAATGAAACGTACACACACTACATTTTCATGA
ACATTATTGCGACCGTTGAGATTCTCATTGTTTGGTGATTGATTATCTAAAGTAGAAGCA
TGAATAGATATAACATAAATGCATAACAAAATGGGTTAGTTATGGGTATACTTCATGCTT
TTCTCTATGTGGTAAAAAAAAATACATGGATCAGCAATGAGTATGATGGTCAATATGGAG
AAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTT
ATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGG
CGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCA
CGTCCAAATGGGGGCTTAGATGAGAAACTTCACGATCGATGCCTTGATTTCGCCATTCCC
AGATACCCATTTCATCTTCAGATTGGTCTGAGATTATGCGAAAATATACACTCATATACA
TAAATACTGACAGTTTGAGCTA
   
Sequence Name 61-K022449-022-241-D05C-8409
GenBank Accession LN510404 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K022449-022-241-D05C-8409
ATAGAGAAAACATGAAGTATACCCATAACTAACCCATTTTGTTATGCATTTATGTTATAT
CTATTCATGCTTCTACTTTAGATAATCAATCACCAAACAATGAGAATCTCAACGGTCGCA
ATAATGTTCATGAAAATGTAGTGTGTGTACGTTTCATTAACTCTCATTTCTTTCTTATTC
TAGTAACTCAACTTCCTCAGTTTCTCTTTAATGTCAACACAATATTTTCGATATTCGCAT
TTAACACACAATACTATTTTTGTCTTCACTTAGAGAAGGAAAAAAAAGAAAGAACAAATT
TAGAAACTTGAGTATAGGGAGAGAGTTGTTTACC
Primers used for PCR MH32 - 8474, show primer details
   
Sequence Name 49-K022450-022-241-D05C-MH32
GenBank Accession LN506695 [GenBank]
Sequencing primer gene specific primer, MH32: 5'-CTCCATCTGGGTAAACAACTCTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K022450-022-241-D05C-MH32
CTCAAGTTTCTAAATTTGTTCTTTCTTTTTTTTCCTTCTCTAAGTGAAGACAAAAATAGT
ATTGTGTGTTAAATGCGAATATCGAAAATATTGTGTTGACATTAAAGAGAAACTGAGGAA
GTTGAGTTACTAGAATAAGAAAGAAATGAGAGTTAATGAAACGTACACACACTACATTTT
CATGAACATTATTGCGACCGTTGAGATTCTCATTGTTTGGTGATTGATTATCTAAAGTAG
AAGCATGAATAGATATAACATAAATGCATAACAAAATGGGTTAGTTATGGGTATACTTCA
TGCTTTTCTCTATGTGGTAAAAAAAAATACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
GCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTT
ATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTAC
ATTCACGTCCAAATGGGGGCTTAGATGAGAAACTTCACGATCGATGCCTTGATTTCGCCA
TTCCCAGATACCCATTTCATCTTCGATTGTCTGAGATATGCGA
   
Sequence Name 53-K022450-022-241-D05C-8409
GenBank Accession LN508015 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K022450-022-241-D05C-8409
GTATTTTTTTTTACCACATAGAGAAAAGCATGAAGTATACCCATAACTAACCCATTTTGT
TATGCATTTATGTTATATCTATTCATGCTTCTACTTTAGATAATCAATCACCAAACAATG
AGAATCTCAACGGTCGCAATAATGTTCATGAAAATGTAGTGTGTGTACGTTTCATTAACT
CTCATTTCTTTCTTATTCTAGTAACTCAACTTCCTCAGTTTCTCTTTAATGTCAACACAA
TATTTTCGATATTCGCATTTAACACACAATACTATTTTTGTCTTCACTTAGAGAAGGAAA
AAAAAGAAAGAACAAATTTAGAAACTTGAGTATAGGGAGAGAGTTGTTTACCCANATGGA
Primers used for PCR BW10 - 8474, show primer details
   
Sequence Name 41-K022451-022-241-D05C-BW10
GenBank Accession LN503926 [GenBank]
Sequencing primer gene specific primer, BW10: 5'-TCCATCTGGGTAAACAACTCTCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K022451-022-241-D05C-BW10
CTCAAGTTTCTAAATTTGTTCTTTCTTTTTTTTCCTTCTCTAAGTGAAGACAAAAATAGT
ATTGTGTGTTAAATGCGAATATCGAAAATATTGTGTTGACATTAAAGAGAAACTGAGGAA
GTTGAGTTACTAGAATAAGAAAGAAATGAGAGTTAATGAAACGTACACACACTACATTTT
CATGAACATTATTGCGACCGTTGAGATTCTCATTGTTTGGTGATTGATTATCTAAAGTAG
AAGCATGAATAGATATAACATAAATGCATAACAAAATGGGTTAGTTATGGGTATACTTCA
TGCTTTTCTCTATGTGGTAAAAAAAAATACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
GCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTT
ATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTAC
ATTCACGTCCAAATGGGGGCTTAGATGAGAAACT
   
Sequence Name 45-K022451-022-241-D05C-8409
GenBank Accession LN505386 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K022451-022-241-D05C-8409
TTTTTTTTTACCACATAGAGAAAAGCATGAAGTATACCCATAACTAACCCATTTTGTTAT
GCATTTATGTTATATCTATTCATGCTTCTACTTTAGATAATCAATCACCAAACAATGAGA
ATCTCAACGGTCGCAATAATGTTCATGAAAATGTAGTGTGTGTACGTTTCATTAACTCTC
ATTTCTTTCTTATTCTAGTAACTCAACTTCCTCAGTTTCTCTTTAATGTCAACACAATAT
TTTCGATATTCGCATTTAACACACAATACTATTTTTGTCTTCACTTAGAGAAGGAAAAAA
AAGAAAGAACAAATTTAGAAACTTGAGTATAGGGAGAGAGTTGTTTACCCAGATGG

Last Updated on 06.12.2018 16:11