Sequences from confirmation amplicon


Confirmation sequences for line 241H02 and hit At1g55500 are shown.
Line ID 241H02
Locus At1g55500 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:20721658
Primers used for PCR 06gz - 8474, show primer details
   
Sequence Name 28-K112307-0022-241-H02C-06gz
GenBank Accession LN498911 [GenBank]
Sequencing primer gene specific primer, 06gz: 5'-GAAAGAGGATGGCTCAATACAGAC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K112307-0022-241-H02C-06gz
GTACAGTTCTTCTTGGAATGAGAACTTGATGGCTTGAATGAACTAAACAGGGGCCCTAGA
GCCAAGGGCACGAAGGCCACAGAAGAGGTTAGCTCAGAGGAGGTGAAAAAGCAGACGTTT
GATGAATCAAATACTGAGGAGACTGTAACATGCGTTCTTCCAGATAGAGAAGAGTGCAAC
AGAGACGATTTTCCAGTTGAGTATAAAGATGCTAAGTTCTTTATCATCAAGTCGTACAGT
GAAGATGATGTGCATAAGAGCATCAAATATAATGTATGGGGTACAGGATATATTCAATTG
TAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATG
GAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 12-K112306-0022-241-H02C-8409
GenBank Accession LN491643 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K112306-0022-241-H02C-8409
TCATTGAATATATCCTGTACCCCATACATTATATTTGATGCTCTTATGCACATCATCTTC
ACTGTACGACTTGATGATAAAGAACTTAGCATCTTTATACTCAACTGGAAAATCGTCTCT
GTTGCACTCTTCTCTATCTGGAAGAACGCATGTTACAGTCTCCTCAGTATTTGATTCATC
AAACGTCTGCTTTTTCACCTCCTCTGAGCTAACCTCTTCTGTGGCCTTCGTGCCCTTGGC
TCTAGGGCCCCTGTTTAGTTCATTCAAGCCATCAATGTTCTCATTTCCATAGAAGTAACT
GTTACCCCTTCCCCGGCTTCTATATTTGTTGTCTGTATTGAGCCATCCTCTTT

Last Updated on 06.12.2018 16:11