Sequences from confirmation amplicon


Confirmation sequences for line 242C01 and hit At3g61415 are shown.
Line ID 242C01
Locus At3g61415 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:22723100
Primers used for PCR 24sm - 8474, show primer details
   
Sequence Name 21-K116610-0022-242-C01C-24sm
GenBank Accession LN495820 [GenBank]
Sequencing primer gene specific primer, 24sm: 5'-ATTTGTAAACCCAATTGTGAGGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K116610-0022-242-C01C-24sm
GTGATGTGAGGCTTTNAAATTATCTTCTTTATATTATTCTTGCGTTAAAATTCAAAGAAC
AAAAAACAGAAAGATAACCAACAAATGAGCCCACCAACTAAATGCTTTAATTAAAAGTTG
TATACAAAAGGTTGATCACTAAAAAGGAGTAAAATAAAAAGAAAACAAAACTGCATCCTA
CCTCTCGGATTAACTGAGAAGAAAGAGAGAAAGCAGATTGTGGAAAGAAAGAAAAATTAC
AGGATGACACACATCAACCGCCGGTGACCGGAACACCACTTTCTCTCTAATTGCTTACAT
TCTAGTGGGGATCTCACCAAAACTGTTCCAGCCATTGGGCTAGCGTTTTCAGCTTCACTT
GTGTCCTGCAGCTGGACCTTCAAAGTTCAAAACGCATGCAAACCAAACCATCTATCATCA
GTCATCTAAAGCTGTGTTGAAGACTCAAGTGTGTCCTATTTCTAAAACCGATAGGTAGTT
CAGTTCAACAGCAATGTACATAAATGGCTACACTATTAACATGTCCGGGATATTTGCGAT
AGGTAGTTCAGTTTCCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTAC
ATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTT
TTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 05-K116609-0022-242-C01C-8409
GenBank Accession LN488513 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K116609-0022-242-C01C-8409
TTGAATATATCCTGGGAACTGAACTACCTATCGCAAATATCCCGGTACATGTTAATAGTG
TAGCCATTTATGTACATTGCTGTTGAACTGAACTACCTATCGGTTTTAGAAATAGGACAC
ACTTGAGTCTTCAACACAGCTTTAGATGACTGATGATAGATGGTTTGGTTTGCATGCGTT
TTGAACTTTGAAGGTCCAGCTGCAGGACACAAGTGAAGCTGAAAACGCTAGCCCAATGGC
TGGAACAGTTTTGGTGAGATCCCCACTAGAATGTAAGCAATTAGAGAGAAAGTGGTGTTC
CGGTCACCGGCGGTTGATGTGTGTCATCCTGTAATTTTTCTTTCTTTCCACAATCTGCTT
TCTCTCTTTCTTCTCAGTTAATCCGAGAGGTAGGATGCAGTTTTGTTTTCTTTTTATTTT
ACTCCTTTTTAGTGATCAACCTTTTGTATACAACTTTTAATTAAAGCATTTAGTTGGTGG
GCTCATTTGTTGGTTATCTTTCTGTTTTTTGTTCTTTGAATTTTAACGCAAGAATAATAT
AAAGAAGATAATTTACAAAGCCTCACATTCACAAGATAAGGAGAGTGGAGGACTCAAAGC
AGCTTCTCCTCACAATTGGTTTTACAAAT

Last Updated on 06.12.2018 16:11