Sequences from confirmation amplicon


Confirmation sequences for line 242C10 and hit At5g03520 are shown.
Line ID 242C10
Locus At5g03520 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:883693
Primers used for PCR 13et - 8474, show primer details
   
Sequence Name 54-K110926-0022-242-C10C-13et
GenBank Accession LN508512 [GenBank]
Sequencing primer gene specific primer, 13et: 5'-CTCATTTTCCATCCAATTTCTTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K110926-0022-242-C10C-13et
GGATAGAACCTCTAGTTGAGACTTAATAAGTTCATCCCACAATCAAATTAGTTGTGGGGT
CAATTGTAACTATTGAAAGTTTCGAGGCTAAAAACTATAAAAACATGAAAATAATATTTT
TCAGCAGCTAATCAACTTGTTTTCTTCAGCGAGCGACATTTTGGAAGGAAGCATTCCATT
AGCTTTTTTGCTGTCTCCAGAAAATAAATTTTCAATCTTTCGTCGGAAAAAAGAAGAAGA
AGAAGCAGAGCTGGGTTTAGTTTTTTCACGAGAAGCAAAGAGATCAATAAGTCAGTGAGC
TTTTTTCTCGTTTCTGACGATGGCGGTTGCGCCGGCNAGAGCTCTGAAGAAACTCAAAAG
TAATGAGTAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTT
TTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 38-K110925-0022-242-C10C-8409
GenBank Accession LN503020 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K110925-0022-242-C10C-8409
CGGCGCACCGCCATCGTCAGAAACGAGAAAAAAGCTCACTGACTTACTGATCTCTTTGCT
TCTCGTGAAAAAACTAAACCCAGCTCTGCTTCTTCTTCTTCTTTTTTCCGACGAAAGATT
GAAAATTTATTTTCTGGAGACAGCAAAAAAGCTAATGGAATGCTTCCTTCCAAAATGTCG
CTCGCTGAAGAAAACAAGTTGATTAGCTGCTGAAAAATATTATTTTCATGTTTTTATAGT
TTTTAGCCTCGAAACTTTCAATAGTTACAATTGACCCCACAACTAATTTGATTGTGGGAT
GAACTTATTAAGTTCTCAACTAAGAGGTTTCTAATCCGGTTTACACCTAGAATCCGACAA
GAAATTGGATGGAAAATGA

Last Updated on 06.12.2018 16:11