Sequences from confirmation amplicon


Confirmation sequences for line 242D07 and hit At2g05380 are shown.
Line ID 242D07
Locus At2g05380 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:1967444
Primers used for PCR GD36 - 8474, show primer details
   
Sequence Name 05-K100850-0022-242-D07C-8409
GenBank Accession LN488275 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K100850-0022-242-D07C-8409
ATCTGTAATTCTTTTTAAATAAGTTAAAACGTTAGATATAAGATACATATAAAATTTGTG
CAAACCATAAATAATGGAGATTGTTTGATATATACGAGTAACACGGATTCATGTTTTGAC
TTAAGTGTCTTTTCTTACGAGTCTTTTGACTTAGCAAGTCCTAATAATTGACCATGGTTC
AAACCAACTTTCTAGATTTAAAACTGTTGAGAGATCAACCGAAAATGAATTAAGATCGCT
AAGACCAAGATTACGAGATTGAGAATTATTTATGGGAATATATCATCCATATTATTAGAA
AAAGAAACGTAATTACTTTCACTGCTAAACATATATACAAGTTTCTATTTGTATTTGTTT
TAGTTTGAGATATATATGTATATTTCCACTTCTGTATAAGTGAATATATTTTGTAACTAC
TATTGACGAAATAGTTTGAAAATATCTAATATCGGTACGTTAGTTTAGGTTTAATTAGCC
TTGGATGTATGAAGTAGACTGAAATATATTTAACACGTACCCGCGGCAGCCATTTCTGAG
ACGATGAAAAGAAATGCAAAGAGACCCAAAAGAAGCAAAGTCTTGGAAGC
   
Sequence Name 05-K100856-0022-242-D07C-GD36
GenBank Accession LN488276 [GenBank]
Sequencing primer gene specific primer, GD36: 5'-TGTAAGAAAATGGCTTCCAAGACT-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K100856-0022-242-D07C-GD36
CGTCTCGAAATGGCTGCCGCGGGTACGTGTTATATATATTTCAGTCTACTTCATACATCC
AAGGCTAATTAAACCTAAACTAACGTACCGATATTAGATATTTTCAAACTATTTCGTCAA
TAGTAGTTACAAAATATATTCACTTATACAGAAGTGGAAATATACATATATATCTCAAAC
TAAAACAAATACAAATAGAAACTTGTATATATGTTTAGCAGTGAAAGTAATTACGTTTCT
TTTTCTAATAATATGGATGATATATTCCCATAAATAATTCTCAATCTCGTAATCTTGGTC
TTAGCGATCTTAATTCATTTTCGGTTGATCTCTCAACAGTTTTAAATCTAGAAAGTTGGT
TTGAACCATGGTCAATTATTAA

Last Updated on 06.12.2018 16:11