Sequences from confirmation amplicon


Confirmation sequences for line 243A07 and hit At1g31390 are shown.
Line ID 243A07
Locus At1g31390 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:11244188
Primers used for PCR CP83 - 8474, show primer details
   
Sequence Name 79-K105358-0022-243-A07C-CP83
GenBank Accession LN513829 [GenBank]
Sequencing primer gene specific primer, CP83: 5'-AAGCGTTTTCACAATTCTCAAGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>79-K105358-0022-243-A07C-CP83
TTAACCTATTTGACGTGAAGTGACTCTACTTATAAAAGTTGTTGTGTGATATTATAAAGT
TTTGTACATGCCACTCGGTTCTCGGATTTATTCTCTTTTCATCTTCTTCTAGTTCTTTTC
TTAGGATTATTTTCTGGACGTTTGGTTGTGACTTGTGAGGAGTCTTCATCAAGAACAATG
GAAGATCAATATGAAAAGAAGATAACATGGACGATTAAGAATTTCTCCTTTGTGCAATCT
CAAGCAATTGATTCTGATATTTTCGTAGTTGGTGACTCCAAATGGTAAAAACTTGTTCTC
TAATGTTTTGTAAGTTTACATTTCATTTTAGGTTTACCTGACATCACCCTTTGCTTATTG
GTTTTTGGTTTATGGTTGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATC
TACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTA
   
Sequence Name 63-K105359-0022-243-A07C-8409
GenBank Accession LN510988 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K105359-0022-243-A07C-8409
ATTTACAATTGAATAATCCTGCAACCATAAACCAAAAACCAATAAGCAAAGGGTGATGTC
AGGTAAACCTAAAATGAAATGTAAACTTACAAAACATTAGAGAACAAGTTTTTACCATTT
GGAGTCACCAACTACGAAAATATCAGAATCAATTGCTTGAGATTGCACAAAGGAGAAATT
CTTAATCGTCCATGTTATCTTCTTTTCATATTGATCTTCCATTGTTCTTGATGAAGACTC
CTCACAAGTCACAACCAAACGTCCAGAAAATAATCCTAAGAAAAGAACTAGAAGAAGATG
AAAAGAGAATAAATCCGAGAACCGAGTGGCATGTACAAAACTTTATAATATCACACAACA
ACTTTTATAAGTAGAGTCACTTCACTGTCAAATAGGTTTGAATGTGAATCGTGTTGATTA
CGAAAACTTGAGAATTGTGAAAACGCT

Last Updated on 06.12.2018 16:11