Sequences from confirmation amplicon


Confirmation sequences for line 243A08 and hit At4g21480 are shown.
Line ID 243A08
Locus At4g21480 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:11434170
Primers used for PCR MR81 - 8474, show primer details
   
Sequence Name 35-K019986-022-243-A08C-MR81
GenBank Accession LN501647 [GenBank]
Sequencing primer gene specific primer, MR81: 5'-GATGCATATGAACAAAACCACAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K019986-022-243-A08C-MR81
GCGTACCATTTTGGTAAAACGCCTGGGGTTCCATCGACACCGAACTTAGCTCCGATCGCC
GCCGCAACAGCTACCTAAACCGTTTACACAATAACCTGATTACTTATCGACCATAATCGA
AATCCAGAAATCGGTTTATAGATGAAGAAATACAAAACCTGAGAAATTAGCATTTGGAAT
CCTCCTTCAAGGAATAGAAATCGTCTTCCCCATTTATCAACGCCATAGATTGACACCACG
GTGGCTCCAACGTTAACCAAACCGGTCACAACCGCCGAAATCAGAGCCGCGTCGCTACCA
AATCCAATCGTTTGAAACAGAACCGGCGCGTAGAACATTATAACATTGATTCCGGTAAGC
TGCTGAAATGCCGGAATCAGAATCGCCATCGTTAGATGTGGTCTATACTTCCTCTGCAAC
AGATTCCTCCATGGATGCTCAACGAGCTTCGAAGCTTCACTGGCGATGATCAAATCGTTG
ATCTCGTCATCAATGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGT
ATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAAT
GTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACG
ATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATT
TCGACGTGTCTACATTC
   
Sequence Name 36-K019986-022-243-A08C-8409
GenBank Accession LN502045 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K019986-022-243-A08C-8409
CATGTAGATTTCCCGGACATGAAGCCATTTACATTGATGACGAGATCAACGATTTGATCA
TCGCCAGTGAAGCTTCGAAGCTCGTTGAGCATCCATGGAGGAATCTGTTGCAGAGGAAGT
ATAGACCACATCTAACGATGGCGATTCTGATTCCGGCATTTCAGCAGCTTACCGGAATCA
ATGTTATAATGTTCTACGCGCCGGTTCTGTTTCAAACGATTGGATTTGGTAGCGACGCGG
CTCTGATTTCGGCGGTTGTGACCGGTTTGGTTAACGTTGGAGCCACCGTGGTGTCAATCT
ATGGCGTTGATAAATGGGGAAGACGATTTCTATTCCTTGAAGGAGGATTCCAAATGCTAA
TTTCTCAGGTTTTGTATTTCTTCATCTATAAACCGATTTCTGGATTTCGATTATGGTCGA
TAAGTAATCAGGTTATTGTGTAAACGGTTTAGGTAGCTGTTGCGGCGGCGATCGGAGCTA
AGTTCGGTGTCGATGGAACCCCAGGCGTTTTACCAAAATGGTACGCCATTGTTGTGGTTT
TGTT

Last Updated on 06.12.2018 16:11