Sequences from confirmation amplicon


Confirmation sequences for line 244C08 and hit At3g60260 are shown.
Line ID 244C08
Locus At3g60260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:22274919
Primers used for PCR 15tn - 8474, show primer details
   
Sequence Name 44-K117029-0022-244-C08C-15tn
GenBank Accession LN505311 [GenBank]
Sequencing primer gene specific primer, 15tn: 5'-ATGAGAGGAGAAACAAGCAATCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K117029-0022-244-C08C-15tn
GAAATGGTACCTGAACAGGCGTTAGATCAAATGAAGGACGAGGGTCACCGTCTCTACCCT
GAACACAAACACAGGAAAGGCCACGGCCTAACCACGCTGCCGATCCAGCTACAACCTCAG
CTGCAATAAAATTAAAATCAGAATTGTGATGTTTTTTGATAAAGAAATGATTTCTTAATG
CAAGAGGGTGACTAGTCAAACATCTTTTAGCTGACTTTTATATTACAAATACGATAACTA
ATACGTGAGAAGCATACAATGTCATTTAAGCATCAGACTTGTCAAAATACAGAACGATGA
AAGAAAGAGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATG
GTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGAT
GTC
   
Sequence Name 12-K117028-0022-244-C08C-8409
GenBank Accession LN491758 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K117028-0022-244-C08C-8409
CTTAAATGACATTGTATGCTTCTCACGTATTAGTTATCGTATTTGTAATATAAAAGTCAG
CTAAAAGATGTTTGACTAGTCACCCTCTTGCATTAAGAAATCATTTCTTTATCAAAAAAC
ATCACAATTCTGATTTTAATTTTATTGCAGCTGAGGTTGTAGCTGGATCGGCAGCGTGGT
TAGGCCGTGGCCTTTCCTGTGTTTGTGTTCAGGGTAGAGACGGTGACCCTCGTCCTTCAT
TTGATCTAACGCCTGCTCAGGTACCATTTTCCTCTTTTTTAATTGGTTTACTTTATAATG
ATTGCTTGTCTTTCCTCTC

Last Updated on 06.12.2018 16:11