Sequences from confirmation amplicon


Confirmation sequences for line 245A02 and hit At4g17200 are shown.
Line ID 245A02
Locus At4g17200 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:9651662
Primers used for PCR CP87 - 8474, show primer details
   
Sequence Name 52-K105459-0022-245-A02C-8409
GenBank Accession LN507817 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K105459-0022-245-A02C-8409
TCGGACTGAGCCATTTACATTGAATATATCCTGAGAAACGGCTTGTACTCTTGCTCAAGA
GAAGATAATTGTGGAAGGAGAAGAAGAAGAAGTAGTAGATGAGATTGAAAAGTTTTTACT
CTGTTTTGATTTTACTACAGAGAGGTTTGGACCGCGTCTTCCTCTGCCATTTCACTCTGA
TGTTTTGGAAACTGTGGCTATATCTTGTGTTAGAGATGATCAGCTTGCGGTGTTATATCA
GCGGTTTGAAACATGGGAGATTTGGGTAACCACTAAGATCGATCCCACTGCGGTGTCCTG
GAGCATGTTTTTGTCAGTGGATATGGAACCACCCACTGGTTGTCAGTTCGATGATGAAGC
TGGGAGCTTCTTTATTGACGAAGAGAACAAAGTCGCTGTATTTTTTAATTCAGACTTGTT
TAACCCAAGAGTAGAAAACCGAAACCGCCGCTACCCGACAGCTTACATCATTGGACAAGA
   
Sequence Name 36-K105458-0022-245-A02C-CP87
GenBank Accession LN502223 [GenBank]
Sequencing primer gene specific primer, CP87: 5'-TCTTGTCCAATGATGTAAGCTGTC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K105458-0022-245-A02C-CP87
GTCTGAATTAAAAATACAGCGACTTTGTTCTCTTCGTCAATAAAGAAGCTCCCAGCTTCA
TCATCGAACTGACAACCAGTGGGTGGTTCCATATCCACTGACAAAAACATGCTCCAGGAC
ACCGCAGTGGGATCGATCTTAGTGGTTACCCAAATCTCCCATGTTTCAAACCGCTGATAT
AACACCGCAAGCTGATCATCTCTAACACAAGATATAGCCACAGTTTCCAAAACATCAGAG
TGAAATGGCAGAGGAAGACGCGGTCCAAACCTCTCTGTAGTAAAATCAAAACAGAGTAAA
AACTTTTCAATCTCATCTACTACTTCTTCTTCTTCTCCTTCCACAATTATCTTCTCTTGA
GCAAGAGTACAAGCCGTTTCTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAA
ATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTAC
CAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATT
Primers used for PCR CP86 - 8474, show primer details
   
Sequence Name 53-K105459-0022-245-A02C-OriL
GenBank Accession LN508154 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K105459-0022-245-A02C-OriL
CGGTATATGGCAGTCGGGACTGACATCAGAACCCTCCATGAATCAGAGCTAAAGTCGTAG
ATTTCGTAACCAAAAAGGTGCCCTGTATCATAGTTCATCAAGATTTTGTAGTTGTGGTTA
TTGTCATATCCGAGAGCGTACCATTCGAGTTTGTTAAACCTTTTTCTTGGTTTGATAAAC
CTTGTTTGCCCTAAATAAGGATTCCACACTAGAAGCCTCGAGTTGTCCTTACCGACGCAT
AACAACAAACCATCGCATTGAAGGACTTTAGATACCTCGATTTGATCAAGTTTAGCAATT
TGCTTTATACATGGATCAACAAAGTCATGTTCGTTGTTTCGGATTCCTTGGAGATCAAAC
TTCATTGAACAAACCCTAGAATCCATCATCGTGAATCCCATAAACTGCTTCCTTGCTGCT
TTAAAGAAAATACCTTCTTTGGATAAAGCGTTCCACATTTTGCAAGTACACCGTACTGAA
ATCAAAGATGTCATCGGAACCCTAGTGAGAATCTCCCCTACCAAATCTGGTGAAAGATCA
GACATTGTCGTCATCCTTTGAAACCTGGCAATAC
   
Sequence Name 37-K105458-0022-245-A02C-CP86
GenBank Accession LN502614 [GenBank]
Sequencing primer gene specific primer, CP86: 5'-TGTATTGCAGAGTTTCAAAGGATG-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K105458-0022-245-A02C-CP86
CACTAGGGTTCCGATGACATCTTTGATTTCAGTACGGTGTACTTGCAAAATGTGGAACGC
TTTATCCAAAGAAGGTATTTTCTTTAAAGCAGCAAGGAAGCAGTTTATGGGATTCACGAT
GATGGATTCTAGGGTTTGTTCAATGAAGTTTGATCTCCAAGGAATCCGAAACAACGAACA
TGACTTTGTTGATCCATGTATAAAGCAAATTGCTAAACTTGATCAAATCGAGGTATCTAA
AGTCCTTCAATGCGATGGTTTGTTGTTATGCGTCGGTAAGGACAACTCGAGGCTTCTAGT
GTGGAATCCTTATTTAGGGCAAACAAGGTTTATCAAACCAAGAAAAAGGTTTAACAAACT
CGAATGGTACGCTCTCGGATATGACAATAACCACAACTACAAAATCTTGATGAACTATGA
TACAGGGCACCTTTTTGGTTACGAAATCTACGACTTTAGCTCTGATTCATGGAGGGTTCT
TGATGTCAGTCCCGACTGCCATATACCGTTTCATGTCCGGGAAATCTACATGGATCAGCA
ATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTC
AAAAATGTAGATGTCCGCACGGTTTATT

Last Updated on 06.12.2018 16:11