Sequences from confirmation amplicon


Confirmation sequences for line 245A04 and hit At4g11150 are shown.
Line ID 245A04
Locus At4g11150 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:6801569
Primers used for PCR SR29 - 8474, show primer details
   
Sequence Name 44-K103582-0022-245-A04C-SR29
GenBank Accession LN505156 [GenBank]
Sequencing primer gene specific primer, SR29: 5'-GAGGAATATGCTGGTAAAGCAAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K103582-0022-245-A04C-SR29
AATATTCCTTCCCCTCCTCCAAATCTAATGATCCTCATGGTCTCCACTGGTAAAATTTCA
TGTTTCTCATCATTACTTTACATTTAAATTTTTATAAAAGAACATTATCAGTGTTTAGAT
CCGGTTTGATAGTGTATTTTGAACATGTAATAGCTCTGGAGGTGTTGTGTTAGCTTCTCG
TGATGGGAAAATCGTGTGCGAGAACACACTTGATGCGCGTCTTGATGTGGCATTCCGTAT
GAAACTCCCAGTGGTATGAAAAACAAATTCTCCAGACTAATCATTGGAGAAAAATACATG
TCCGGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAAT
GAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAA
AAATGTAGATGTCCG
   
Sequence Name 60-K103583-0022-245-A04C-8409
GenBank Accession LN510236 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K103583-0022-245-A04C-8409
GGACAGTATTTTTTCTCCAATGATTAGTCTGGAGAATTTGTTTTTCATACCACTGGGAGT
TTCATACGGAATGCCACATCAAGACGCGCATCAAGTGTGTTCTCGCACACGATTTTCCCA
TCACGAGAAGCTAACACAACACCTCCAGAGCTATTACATGTTCAAAATACACTATCAAAC
CGGATCTAAACACTGATAATGTTCTTTTATAAAAATTTAAATGTAAAGTAATGATGAGAA
ACATGAAATTTTACCAGTGGAGACCATGAGGATCATTAGATTTAGGAGGAGGGGGAAGGA
ATATTTTTGTGTCAACAGCAACCTCTGGGGCATGAACCTTTGCTTTACCACATTTATTCC

Last Updated on 06.12.2018 16:11