Sequences from confirmation amplicon


Confirmation sequences for line 245D04 and hit At3g05010 are shown.
Line ID 245D04
Locus At3g05010 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:1389888
Primers used for PCR 04ey - 8474, show primer details
   
Sequence Name 53-K111269-0022-245-D04C-04ey
GenBank Accession LN508188 [GenBank]
Sequencing primer gene specific primer, 04ey: 5'-TAATATCTTTTTGGTGGGCAAAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K111269-0022-245-D04C-04ey
AAGAATACACATTATTGATTATTAAATAGCACTTCAAAAATTTACATTTCATAAGATTGA
TTGATTTAATAAGTCTTTCAAATTTAAGATACAGAATTGAAAAGAATAATATTTAAATTC
TTTAAACATCACATGTCCAATAATTAACTCTTTAAACATCACATGTAAAATCAGTTAAAA
ATAAAAAGGATGAGAAAAATGATAGTTTTTTTTTTTTTTTTAAAAGGGAAAGGGGAAAAA
CCAATAAAATTTCCCTATAAACCCCTTAAATTTTGGGGAAATTCCCCAGGTTGGCTCCAA
GTGTAGGAATTGGGCCCCCAAAAAAAAAAAAAAATTTCCAATTCAACCAATTTCTGTTTC
GAAAAACCTTCAAATCTCAAATTTGACCCCCTTTTCGGAAACAAGGAATGGTTTCCCCCC
CCTGGCCCAAATCCAACTGGGTTCCGAATCCGAACAATTGAAAAAAAGGCAAGGGCCCAC
CAAAATGGCGAATCCCCCTTTCGGAATTTCCCGGCAAACCCCGAATTCAACGGCCCCCGA
AGGTTTTATCGCCGAAGGGTTCGGAAATTGTCAGGGTTTTCTCCAAAACAGGGTTTGGGT
TTAACCACCTTTCCAATCCGCCCCTAACTAACCGAACAAGGCAAAAAAAACTTGGCCCAA
TTTGTCAAACCGAAAATCTAACTTAAGAATGGCCAATAAGGGTTTTCTTGGCTTGGTAAC
TTGGCCAAACCTGGCTGGGGGTGCCCTCCAGAAAATTTCCATTGGAAAAGGCCTCCAGGC
CGGGAAAACCTACTGGAATCACC
   
Sequence Name 37-K111268-0022-245-D04C-8409
GenBank Accession LN502654 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K111268-0022-245-D04C-8409
CATTTACATTGAATTATCCTGAAGGCACACCAAGCAAGATTGAGCAAGCTAACAAGCCAA
AGAAAACCATAATAGGCAATCATAATGTAAGATCTCCGATTAGACAACTTGGACAAGCTC
TTCTTCGCCTCGTACGCTAAGTAAGCGACGAATAGAATAGATGCTAAAACCAAAACCGTG
TTATGAAGAAACCCATGACACTTCCCGACCCATCCGCCGATAAACCCTCCGGTGGCCGTC
GAATCAGGGCTTAGCCGAGAAATCACGAACGGTGACTCCGCAATCTCGCTGAGCACTCGC
ATTTCATCAAATTGTACGAATCAGAAACCAAGTAGACTTAGCGCAAGGAGCGGAAAGCAA
TCCCTAGTTCCAGAAAAGGAGCTCAATTTCGAGATTTGATGTTTTTGCGATACAGAATTC
GTATGAATCGTATATTTCTTCTTCTCTCTGGCGACCAAATCCATACACTTGACGCAAACA
TGAGTAATTACACAAAAGTTAAAGGGTATTATAGGTAATTATCATCGCTTCTCCCACTTC
CACTCTAAAACAAAAAAAAAAACCTACCATTTTTCCCACCCTTTTAATTTTAAACGGATT
TAACTGGGAAGGTTAAAAAATTTATTTTTGGAACTGGGGAGGTTAAAAAAATTAAAATAT
AATCCTTTCCATTTCGGAATCTAAATTTGAAAAAATTTTTAAATTCATTCATTCTAAGAA
ATGGAAATTTTTGAAGGGGCTTTTAAAAAACCAATAAGGGGTATTCCTTTTTAATAAAAT
AAAAAAACGGGGAAAACATTGGTCTTTCTTTGGCCACAAAAAAA

Last Updated on 06.12.2018 16:11