Sequences from confirmation amplicon


Confirmation sequences for line 245E01 and hit At5g25410 are shown.
Line ID 245E01
Locus At5g25410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:8833214
Primers used for PCR 39mk - 8474, show primer details
   
Sequence Name 11-K114404-0022-245-E01C-8409
GenBank Accession LN491215 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K114404-0022-245-E01C-8409
ATTTCATTGAATAATCCTGGAGGACTTGGACCTTGATGAGAAGCTTGAACTGCACCTCCA
ACTCCCACCATATTTGCACCATTACCTATTAGGTTAAATAATTCTTTTGGCCAATATCCG
ACATCTATGTTAGGTGCATTTGCTATCAGTTTTGTAATCCACCAGTTTCCTGTTTGTTTA
TCCTTTTTGTGTATAAGAAAATAATCATAATAAAAAAATTAAAAATTGAAGAATGAAATA
GCATAAAATATTTAAAAATGAAAAACATAAAATATTTAAATCTTGAAAAATTACCTGATG
GATGGAGTAATGAANCAAATCT
   
Sequence Name 33-K114405-0022-245-E01C-39mk
GenBank Accession LN501090 [GenBank]
Sequencing primer gene specific primer, 39mk: 5'-AAGATTTGTTGCATTACTCCATCC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K114405-0022-245-E01C-39mk
GATTATTTTATGTTTTTCATTTTTAATATTTTATGCTATTTCATTCTTCAATTTTTAATT
TTTTTATTATGATTATTTTCTTATACACAAAAAGGATAAACAAACAGGAAACTGGTGGAT
TACAAAACTGATAGCAAATGCACCTAACATAGATGTCGGATATTGGCCAAAAGAATTATT
TAACCTAATAGGTAATGGTGCAAATATGGTGGGAGTTGGAGGTGCAGTTCAAGCTTCTCA
TCAAGGTCCAAGTCCTCCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCT
ACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAAT
TTTTTTTCAATTCAAAAATGTAGATGTCCGCAG

Last Updated on 06.12.2018 16:11