Sequences from confirmation amplicon


Confirmation sequences for line 245E12 and hit At5g11020 are shown.
Line ID 245E12
Locus At5g11020 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:3486993
Primers used for PCR 22ac - 8474, show primer details
   
Sequence Name 38-K115837-0022-245-E12C-22ac
GenBank Accession LN503104 [GenBank]
Sequencing primer gene specific primer, 22ac: 5'-GGGGATTAGAATATTTGCATGAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K115837-0022-245-E12C-22ac
CACATCTTATTAGATAGCAATTTCAATGCTAAGGTAAACAAATCAGTGATTAAAGTCATA
ATGTTTCAATATCATATTCCTTTGTTTTTTTTGTCTAAAGCTCTAGCTTTCTATGAAATG
TAGATTTCAGATTTTGGTCTAGCTGTTGTTGATGGGCCAAAGAACAAGAACCATAAACTT
TCCGGGACAGTTGGCTACGTTGCACCAGAGTATCTTCTCAACGGTAACACCCTGAAATAA
CAATCTGTCAAGTCTTACTTTAAAAAAAAATCATCCTTAAAGAAACTTGTTGTGTTTTTA
TGAGAAGCATTTCTTACTCATCACCATACTGCATGTGTTTGTTTCAATTGTAAATGGCTT
CATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 06-K115833-0022-245-E12C-8409
GenBank Accession LN488956 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K115833-0022-245-E12C-8409
TGAACAACACATGCAGTATGGTGATGAGTAAGAAATGCTTCTCATAAAAACACAACAAGT
TTCTTTAAGGATGATTTTTTTTTAAAGTAAGACTTGACAGATTGTTATTTCAGGGTGTTA
CCGTTGAGAAGATACTCTGGTGCAACGTAGCCAACTGTCCCGGAAAGTTTATGGTTCTTG
TTCTTTGGCCCATCAACAACAGCTAGACCAAAATCTGAAATCTACATTTCATAGAAAGCT
AGAGCTTTAGACAAAAAAAACAAAGGAATATGATATTGAAACATTATGACTTTAATCACT
GATTTGTTTACCTTAGCATTGAAATTGCTATCTAATAAGATGTTGGATGATTTCAAGTCC
CTGTGAATGATTGCTGGATGACAATGTTCATGCAAATATCATAATCCC

Last Updated on 06.12.2018 16:11