Sequences from confirmation amplicon


Confirmation sequences for line 245G06 and hit At4g11480 are shown.
Line ID 245G06
Locus At4g11480 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:6972581
Primers used for PCR 02fr - 8474, show primer details
   
Sequence Name 37-K117317-0022-245-G06C-02fr
GenBank Accession LN502760 [GenBank]
Sequencing primer gene specific primer, 02fr: 5'-GTACCGTCTTTTCCAATCTAGCTG-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K117317-0022-245-G06C-02fr
TATTTTTAAAACATTTGGTAAAATGCACATAATTAAACCAAGTAAGTGTTTTTGTTTATT
ACCAAAGAGGAAATAATTAAGGCTCTTGTTGGGAACAAACTCGTAGACAAGTATCTGTTC
ATCTCTTTCTAAGCAAAACCCAAGAAGCCTAACAAGATTCTTATGTTGAAGCTTAGCCAC
AATTACAACCTCGTTCTTGAACTCTTGTGTGCCTTGTCCTGAATTACTCGATAGCCGCTT
CACCGCAACTTCTGTTTCGTTTGGTAACATTCCCTACAAATATACAATATTACAATTACT
GACGAATGAAACCAAAGTTGCGGTGAAGCTTCAATTGTAAATGGCTTCATGTCCGGGAAA
TCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACC
AATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 28-K117316-0022-245-G06C-8409
GenBank Accession LN499018 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K117316-0022-245-G06C-8409
GCTTCACCGCAACTTTGGTTTCATTCGTCAGTAATTGTAATATTGTATATTTGTAGGGAA
TGTTACCAAACGAAACAGAAGTTGCGGTGAAGCGGCTATCGAGTAATTCAGGACAAGGCA
CACAAGAGTTCAAGAACGAGGTTGTAATTGTGGCTAAGCTTCAACATAAGAATCTTGTTA
GGCTTCTTGGGTTTTGCTTAGAAAGAGATGAACAGATACTTGTCTACGAGTTTGTTCCCA
ACAAGAGCCTTAATTATTTCCTCTTTGGTAATAAACAAAAACACTTACTTGGTTTAATTA
TGTGCATTTTACCAAATGTTTTTAAAAATATTTGTCTTATGCAGATCCAACCAAGAAGAG
TCAGCTAGATTGGAAAGGACGGTA

Last Updated on 06.12.2018 16:11