Sequences from confirmation amplicon


Confirmation sequences for line 246D10 and hit At1g07430 are shown.
Line ID 246D10
Locus At1g07430 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:2281302
Primers used for PCR GD30 - 8474, show primer details
   
Sequence Name 08-K100850-0022-246-D10C-8409
GenBank Accession LN489658 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K100850-0022-246-D10C-8409
GCCATTTACATTGAATTATCCTGAAAGAACGGGAAGAGAGGAGAGATCACGGACAAAGCA
TGTACGGAGGCGTCAGTATTGCTGACGAAGCTGGCGTTGGCAAAACACAGTAGTGACAAC
GTAAGCGTCGTCGTTATTGATCTCAGAAGAAGAAGAAAGAGACACGTTGCTTGACACTAA
TCTCCTCATCACTCTTACTCCACCGTTTTTTGTGTTGTTTAAAATGATTCTTTTACCTTG
GGACGAAGGTAGCTCAAATCTTTGAACCCAAAAGAGAGAAAAAAAAAGCATTAGGTCTAA
AAAAAAAACCCCGGTTTACTGAAAGCGGTTAAACCGGGTATGGTTGGGTCTTTTTTGTTC
TGAACCTTTTTTTCTTGCCTTTTTTCGGTGTGTAATGAACAAACAGAAAAGGAAGCCGGA
ATGTGTCACTGTCTAGGTGGAATCAGTTCAGAATTCA
   
Sequence Name 08-K100856-0022-246-D10C-GD30
GenBank Accession LN489659 [GenBank]
Sequencing primer gene specific primer, GD30: 5'-ATTGAATTCTGAACTGATTCCACC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K100856-0022-246-D10C-GD30
TTCGGCTTCTTTTCGTTTGTTCATTACACACCGAAAAAAGGCAAGAAAAAAAGGTTCAGA
ACAAAAAAGACCCAACCATACCCGGTTTAACCGCTTTCAGTAAACCGGGGTTTTTTTTTT
AGACCTAAGGCTTTTTTTTTCTCTCTTTGGGGTTCAAAGATTTGAGCTACCTTCGTCCCA
AGGTAAAAGAATCATTTTAAACAACACAAAAAACGGTGGAGTAAGAGTGATGAGGAGATT
AGTGTCAAGCAACGTGTCTCTTTCTTCTTCTTCTGAGATCAATAACGACAACCCTAACGT
TGTCACTACGGTGTTTTGCCAACGCCAGCTTCGTCAGCAATACTGACGCCTCCGTACATG
CTTTGTCCGTGATCTCTCCTCTCTTCCCGTTCTTTCAGGATATATTCAATTGTAAATGGC
TTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAA
GAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATTTCCC

Last Updated on 06.12.2018 16:11